ArticleRNA (New York, N.Y.)2011
The stoichiometry of the Escherichia coli Hfq protein bound to RNA.
Article in RNA (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Dynamic Refolding of OxyS sRNA by the Hfq RNA Chaperone.Journal of molecular biology · 2022Article
- Fluorescent Nanoparticles Coated with a Somatostatin Analogue Target Blood Monocyte for Efficient Leukaemia Treatment.Pharmaceutical research · 2020Article
- Inverted Regulation of Multidrug Efflux Pumps, Acid Resistance, and Porins in Benzoate-Evolved Escherichia coli K-12.Applied and environmental microbiology · 2019Article
- Targeting of somatostatin receptors expressed in blood cells using quantum dots coated with vapreotide.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2018Article
- Post-transcriptional gene regulation by an Hfq-independent small RNA in Caulobacter crescentus.Nucleic acids research · 2018Article
- Intermolecular base stacking mediates RNA-RNA interaction in a crystal structure of the RNA chaperone Hfq.Scientific reports · 2017Article
- RNA search engines empower the bacterial intranet.Biochemical Society transactions · 2017Review
- Structural and Biochemical Characterization of Poly-ADP-ribose Polymerase from Trypanosoma brucei.Scientific reports · 2017Article
- Crystal structure and RNA-binding properties of an Hfq homolog from the deep-branching Aquificae: conservation of the lateral RNA-binding mode.Acta crystallographica. Section D, Structural biology · 2017Article
- Characterization of the DNA dependent activation of human ARTD2/PARP2.Scientific reports · 2016Article
- Structural insights into the recognition of the internal A-rich linker from OxyS sRNA by Escherichia coli Hfq.Nucleic acids research · 2015Article
- Fluorescence reporters for Hfq oligomerization and RNA annealing.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes.Current opinion in biotechnology · 2014Review
- Near-infrared quantum dots for HER2 localization and imaging of cancer cells.International journal of nanomedicine · 2014Article
- Duplex formation between the sRNA DsrA and rpoS mRNA is not sufficient for efficient RpoS synthesis at low temperature.RNA biology · 2013Article
- RNA-seq reveals the RNA binding proteins, Hfq and RsmA, play various roles in virulence, antibiotic production and genomic flux in Serratia sp. ATCC 39006.BMC genomics · 2013Article
- Identifying and characterizing Hfq-RNA interactions.Methods (San Diego, Calif.) · 2013Review
- Hfq binding changes the structure ofRNA (New York, N.Y.) · 2013Article
- Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA.Nucleic acids research · 2013Article
- Cycling of RNAs on Hfq.RNA biology · 2013Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Escherichia coli RNA binding protein Hfq is involved in many aspects of post-transcriptional gene expression. Tight binding of Hfq to polyadenylate sequences at the 3' end of mRNAs influences exonucleolytic degradation, while Hfq binding to small noncoding RNAs (sRNA) and their targeted mRNAs facilitate their hybridization which in turn effects translation. Hfq binding to an A-rich tract in the 5' leader region of the rpoS mRNA and to the sRNA DsrA have been shown to be important for DsrA enhanced translation initiation of this mRNA. The complexes of Hfq-A(18) and Hfq-DsrA provide models for understanding how Hfq interacts with these two RNA sequence/structure motifs. Different methods have reported different values for the stoichiometry of Hfq-A(18) and Hfq-DsrA. In this work, mass spectrometry and analytical ultracentrifugation provide direct evidence that the strong binding mode of the Hfq hexamer (Hfq(6)) for A(18) and domain II of DsrA (DsrA(DII)) involve 1:1 complexes. This stoichiometry was also supported by fluorescence anisotropy and a competition gel mobility shift experiment using wild-type and truncated Hfq. More limited studies of Hfq binding to DsrA as well as to the sRNAs RprA, OxyS, and an 18-nt segment of OxyS were also consistent with 1:1 stoichiometry. Mass spectrometry of cross-linked samples of Hfq(6), A(18), and DsrA(DII) exhibit intensity corresponding to a ternary 1:1:1 complex; however, the small intensity of this peak and fluorescence anisotropy experiments did not provide evidence that this ternary complex is stable in solution.
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