ArticleThe Journal of biological chemistry2011
Nuclear/nucleolar GTPase 2 proteins as a subfamily of YlqF/YawG GTPases function in pre-60S ribosomal subunit maturation of mono- and dicotyledonous plants.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 28 citations in OpenAlex.
- Arabidopsis EMB2788/Npa1L1 is critical for pre-rRNA processing, ribosome biogenesis, and embryo development.The Plant journal : for cell and molecular biology · 2026Article
- Proteomic Analysis Reveals Accompanying Anion-Dependent Changes of Cadmium Toxicity During Arabidopsis thaliana Development.Plant, cell & environment · 2026Article
- Ribosome biogenesis in plants requires the nuclear envelope and mitochondria localized OPENER complex.Nature communications · 2025Article
- Identification of genetic loci for growth and stem form traits in hybridForestry research · 2025Article
- Proteomic profiling of Arabidopsis nuclei reveals distinct protein accumulation kinetics upon heat stress.Scientific reports · 2024Article
- The nucleolar protein NOL12 is required for processing of large ribosomal subunit rRNA precursors in Arabidopsis.BMC plant biology · 2023Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2022Article
- The nucleolar protein SAHY1 is involved in pre-rRNA processing and normal plant growth.Plant physiology · 2021Article
- Ribosome Biogenesis in Plants: From Functional 45S Ribosomal DNA Organization to Ribosome Assembly Factors.The Plant cell · 2019Review
- Functional characterization of chloroplast-targeted RbgA GTPase in higher plants.Plant molecular biology · 2017Article
- The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes.Journal of experimental botany · 2015Article
- The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis.Journal of experimental botany · 2015Article
- Identification and Expression Analysis of Ribosome Biogenesis Factor Co-orthologs in Solanum lycopersicum.Bioinformatics and biology insights · 2015Article
- Functional interaction between ribosomal protein L6 and RbgA during ribosome assembly.PLoS genetics · 2014Article
- Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.Journal of experimental botany · 2014Article
- DER containing two consecutive GTP-binding domains plays an essential role in chloroplast ribosomal RNA processing and ribosome biogenesis in higher plants.Journal of experimental botany · 2014Article
- Mutational analysis of the ribosome assembly GTPase RbgA provides insight into ribosome interaction and ribosome-stimulated GTPase activation.Nucleic acids research · 2013Article
- 40S ribosome biogenesis co-factors are essential for gametophyte and embryo development.PloS one · 2013Article
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8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The YlqF/YawG families are important GTPases involved in ribosome biogenesis, cell proliferation, or cell growth, however, no plant homologs have yet to be characterized. Here we isolated rice (Oryza sativa) and Arabidopsis nuclear/nucleolar GTPase 2 (OsNug2 and AtNug2, respectively) that belong to the YawG subfamily and characterized them for pre-60S ribosomal subunit maturation. They showed typical intrinsic YlqF/YawG family GTPase activities in bacteria and yeasts with k(cat) values 0.12 ± 0.007 min(-1) (n = 6) and 0.087 ± 0.002 min(-1) (n = 4), respectively, and addition of 60S ribosomal subunits stimulated their activities in vitro. In addition, OsNug2 rescued the lethality of the yeast nug2 null mutant through recovery of 25S pre-rRNA processing. By yeast two-hybrid screening five clones, including a putative one of 60S ribosomal proteins, OsL10a, were isolated. Subcellular localization and pulldown assays resulted in that the N-terminal region of OsNug2 is sufficient for nucleolar/nuclear targeting and association with OsL10a. OsNug2 is physically associated with pre-60S ribosomal complexes highly enriched in the 25S, 5.8S, and 5S rRNA, and its interaction was stimulated by exogenous GTP. Furthermore, the AtNug2 knockdown mutant constructed by the RNAi method showed defective growth on the medium containing cycloheximide. Expression pattern analysis revealed that the distribution of AtNug2 mainly in the meristematic region underlies its potential role in active plant growth. Finally, it is concluded that Nug2/Nog2p GTPase from mono- and didicotyledonous plants is linked to the pre-60S ribosome complex and actively processed 27S into 25S during the ribosomal large subunit maturation process, i.e. prior to export to the cytoplasm.
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