ArticleDevelopment (Cambridge, England)2011
Xenopus germline nanos1 is translationally repressed by a novel structure-based mechanism.
Article in Development (Cambridge, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- A single cell RNA sequencing resource for early sea urchin development.Development (Cambridge, England) · 2020Article
- Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.Developmental biology · 2019Article
- Novel functions of the ubiquitin-independent proteasome system in regulatingDevelopment (Cambridge, England) · 2019Article
- Combined functions of two RRMs in Dead-end1 mimic helicase activity to promote nanos1 translation in the germline.Molecular reproduction and development · 2018Article
- Maternal Dead-end 1 promotes translation ofDevelopment (Cambridge, England) · 2017Article
- Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin.Developmental biology · 2016Article
- Hermes (Rbpms) is a Critical Component of RNP Complexes that Sequester Germline RNAs during Oogenesis.Journal of developmental biology · 2016Article
- The Xenopus Maternal-to-Zygotic Transition from the Perspective of the Germline.Current topics in developmental biology · 2015Review
- Every which way--nanos gene regulation in echinoderms.Genesis (New York, N.Y. : 2000) · 2014Review
- Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin.Developmental biology · 2013Article
- A single Drosophila embryo extract for the study of mitosis ex vivo.Nature protocols · 2013Article
- The forkhead transcription factor FoxY regulates Nanos.Molecular reproduction and development · 2012Article
- Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells.Development (Cambridge, England) · 2012Article
- Nanos1 functions as a translational repressor in the Xenopus germline.Mechanisms of developmentArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
The translational repressor Nanos is expressed in the germline and stem cell populations of jellyfish as well as humans. Surprisingly, we observed that unlike other mRNAs, synthetic nanos1 RNA translates very poorly if at all after injection into Xenopus oocytes. The current model of simple sequestration of nanos1 within germinal granules is insufficient to explain this observation and suggests that a second level of repression must be operating. We find that an RNA secondary structural element immediately downstream of the AUG start site is both necessary and sufficient to prevent ribosome scanning in the absence of a repressor. Accordingly, repression is relieved by small in-frame insertions before this secondary structure, or translational control element (TCE), that provide the 15 nucleotides required for ribosome entry. nanos1 is translated shortly after fertilization, pointing to the existence of a developmentally regulated activator. Oocyte extracts were rendered fully competent for nanos1 translation after the addition of a small amount of embryo extract, confirming the presence of an activator. Misexpression of Nanos1 in oocytes from unlocalized RNA results in abnormal development, highlighting the importance of TCE-mediated translational repression. Although found in prokaryotes, steric hindrance as a mechanism for negatively regulating translation is novel for a eukaryotic RNA. These observations unravel a new mode of nanos1 regulation at the post-transcriptional level that is essential for normal development.
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