ArticlePlant physiology2011
Distribution of callose synthase, cellulose synthase, and sucrose synthase in tobacco pollen tube is controlled in dissimilar ways by actin filaments and microtubules.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 160 citations in OpenAlex.
- Review
- Low-temperature stress modulates pollen tube growth through temperature-dependent multi-level regulatory mechanisms in Camellia sinensis.Plant reproduction · 2026Article
- Microtubules in Arabidopsis pollen tubes are oriented away from the tube apex and are actin-independent at the cortex.bioRxiv : the preprint server for biology · 2026Article
- β-Glucan Synthesis Gene Family in Crops: Advances in Classification, Function, and Regulation.Current issues in molecular biology · 2025Review
- Effect of UV-B stress on olive (Olea europaea L.) pollen tubes: A study of callose plug deposition and male germ unit integrity.Protoplasma · 2025Article
- PbrBGAL6 promotes pollen tube growth by influencing apical pectin level in Pyrus bretschneideri.BMC genomics · 2025Article
- An involvement of a new zinc finger protein PbrZFP719 into pear self-incompatibility reaction.Plant cell reports · 2025Article
- Chitosan stimulates root hair callose deposition, endomembrane dynamics, and inhibits root hair growth.Plant, cell & environment · 2025Article
- Sucrose synthase dynamics and its potential role in heat stress tolerance in cereals.Frontiers in plant science · 2025Review
- A high-density linkage map and fine QTL mapping of architecture, phenology, and yield-related traits in faba bean (Frontiers in plant science · 2025Article
- Plasmodesmata Function and Callose Deposition in Plant Disease Defense.Plants (Basel, Switzerland) · 2024Review
- Article
- Arabidopsis cyclophilins direct intracellular transport of mobile mRNA via organelle hitchhiking.Nature plants · 2024Article
- Microtubule-associated proteins WDL5 and WDL6 play a critical role in pollen tube growth inPlant signaling & behavior · 2023Article
- Pollen tube invasive growth is promoted by callose.Plant reproduction · 2023Article
- Genetic Regulation of Mitosis-Meiosis Fate Decision in Plants: Is Callose an Oversighted Polysaccharide in These Processes?Plants (Basel, Switzerland) · 2023Review
- Membrane Dynamics Regulated by Cytoskeleton in Plant Immunity.International journal of molecular sciences · 2023Review
- VaSUS2 confers cold tolerance in transgenic tomato and Arabidopsis by regulation of sucrose metabolism and ROS homeostasis.Plant cell reports · 2023Article
- Haplotype variations of sucrose phosphate synthase B gene among sugarcane accessions with different sucrose content.BMC genomics · 2023Article
- Expression of Clementine Asp-Rich Proteins (CcASP-RICH) in Tobacco Plants Interferes with the Mechanism of Pollen Tube Growth.International journal of molecular sciences · 2022Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Callose and cellulose are fundamental components of the cell wall of pollen tubes and are probably synthesized by distinct enzymes, callose synthase and cellulose synthase, respectively. We examined the distribution of callose synthase and cellulose synthase in tobacco (Nicotiana tabacum) pollen tubes in relation to the dynamics of actin filaments, microtubules, and the endomembrane system using specific antibodies to highly conserved peptide sequences. The role of the cytoskeleton and membrane flow was investigated using specific inhibitors (latrunculin B, 2,3-butanedione monoxime, taxol, oryzalin, and brefeldin A). Both enzymes are associated with the plasma membrane, but cellulose synthase is present along the entire length of pollen tubes (with a higher concentration at the apex) while callose synthase is located in the apex and in distal regions. In longer pollen tubes, callose synthase accumulates consistently around callose plugs, indicating its involvement in plug synthesis. Actin filaments and endomembrane dynamics are critical for the distribution of callose synthase and cellulose synthase, showing that enzymes are transported through Golgi bodies and/or vesicles moving along actin filaments. Conversely, microtubules appear to be critical in the positioning of callose synthase in distal regions and around callose plugs. In contrast, cellulose synthases are only partially coaligned with cortical microtubules and unrelated to callose plugs. Callose synthase also comigrates with tubulin by Blue Native-polyacrylamide gel electrophoresis. Membrane sucrose synthase, which expectedly provides UDP-glucose to callose synthase and cellulose synthase, binds to actin filaments depending on sucrose concentration; its distribution is dependent on the actin cytoskeleton and the endomembrane system but not on microtubules.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.