ArticleNeuron glia biology2010
Monitoring astrocyte calcium microdomains with improved membrane targeted GCaMP reporters.
Article in Neuron glia biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
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Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Cell adhesion and intracellular calcium signaling in neurons.Cell communication and signaling : CCS · 2013Pooled it
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- Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.Communications biology · 2025Article
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- Astrocyte's self-repairing characteristics improve working memory in spiking neuronal networks.iScience · 2023Article
- Photoswitching alters fluorescence readout of jGCaMP8 CaProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Toolbox for studying neurovascular couplingNeurophotonics · 2022Review
- MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells.Cell reports · 2021Article
- Extracellular Calcium Influx Pathways in Astrocyte Calcium Microdomain Physiology.Biomolecules · 2021Review
- Mechanical and chemical activation of GPR68 probed with a genetically encoded fluorescent reporter.Journal of cell science · 2021Article
- Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging.STAR protocols · 2021Article
- Interactions of neuroimmune signaling and glutamate plasticity in addiction.Journal of neuroinflammation · 2021Review
- Potential and Realized Impact of Astroglia CaFrontiers in cellular neuroscience · 2021Review
- High-Resolution Three-Dimensional Imaging of Individual Astrocytes Using Confocal Microscopy.Current protocols in neuroscience · 2020Article
- Post-treatment with Posiphen Reduces Endoplasmic Reticulum Stress and Neurodegeneration in Stroke Brain.iScience · 2020Article
- A centrosome-localized calcium signal is essential for mammalian cell mitosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
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3 authors.
Funding
Abstract
Astrocytes are involved in synaptic and cerebrovascular regulation in the brain. These functions are regulated by intracellular calcium signalling that is thought to reflect a form of astrocyte excitability. In a recent study, we reported modification of the genetically encoded calcium indicator (GECI) GCaMP2 with a membrane-tethering domain, Lck, to generate Lck-GCaMP2. This GECI allowed us to detect novel microdomain calcium signals. The microdomains were random and 'spotty' in nature. In order to detect such signals more reliably, in the present study we further modified Lck-GCaMP2 to carry three mutations in the GCaMP2 moiety (M153K, T203V within EGFP and N60D in the CaM domain) to generate Lck-GCaMP3. We directly compared Lck-GCaMP2 and Lck-GCaMP3 by assessing their ability to monitor several types of astrocyte calcium signals with a focus on spotty microdomains. Our data show that Lck-GCaMP3 is between two- and four-times better than Lck-GCaMP2 in terms of its basal fluorescence intensity, signal-to-noise and its ability to detect microdomains. The use of Lck-GCaMP3 thus represents a significantly improved way to monitor astrocyte calcium signals, including microdomains, and will facilitate detailed exploration of their molecular mechanisms and physiological roles.
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