ArticleProtein & cell2010
Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases.
Article in Protein & cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 67 citations in OpenAlex.
- Elucidating the Multi-Enzymatic Mechanism of Bacterial Decolorization of Azo and Indigoid Dyes: An Integrated Study of Degradation Pathways and Molecular Docking.International journal of molecular sciences · 2026Article
- Bidirectional Interplay Between IBD Therapies and the Gut Microbiota: A Pharmacomicrobiomic Approach to Personalized Treatment.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2025Review
- Cross-Kingdom Enzymatic Strategies for Deoxynivalenol Detoxification: Computational Analysis of Structural Mechanisms and Evolutionary Adaptations.Microorganisms · 2025Article
- Supramolecular discrimination and diagnosis-guided treatment of intracellular bacteria.Nature communications · 2025Article
- Sequence similarity network analysis of drug- and dye-modifying azoreductase enzymes found in the human gut microbiome.Archives of biochemistry and biophysics · 2024Article
- Genome-Scale Investigation of the Regulation of azoR Expression in Escherichia coli Using Computational Analysis and Transposon Mutagenesis.Microbial ecology · 2024Article
- Gut Microbiome-Wide Search for Bacterial Azoreductases Reveals Potentially Uncharacterized Azoreductases Encoded in the Human Gut Microbiome.Drug metabolism and disposition: the biological fate of chemicals · 2023Article
- Lactose azocalixarene drug delivery system for the treatment of multidrug-resistant pseudomonas aeruginosa infected diabetic ulcer.Nature communications · 2022Article
- Gut microbiota mediates the absorption of FLZ, a new drug for Parkinson's disease treatment.Acta pharmaceutica Sinica. B · 2021Article
- Gut microbiota-driven drug metabolism in inflammatory bowel disease.Journal of Crohn's & colitis · 2021Article
- An Integrative Approach to Study Bacterial Enzymatic Degradation of Toxic Dyes.Frontiers in microbiology · 2021Review
- Mechanistic and Crystallographic Studies of Azoreductase AzoA fromACS chemical biology · 2020Article
- Ammonia-oxidising archaea living at low pH: Insights from comparative genomics.Environmental microbiology · 2017Article
- Azoreductases in drug metabolism.British journal of pharmacology · 2017Review
- Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity.BMC microbiology · 2017Article
- Functional Annotation of a Presumed Nitronate Monoxygenase Reveals a New Class of NADH:Quinone Reductases.The Journal of biological chemistry · 2016Article
- 16SrRNA sequencing of Dye decolorizing bacteria isolated from Soil.Bioinformation · 2015Article
- Comparison of Metabolic Pathways in Escherichia coli by Using Genetic Algorithms.Computational and structural biotechnology journal · 2015Article
- Arylamine N-acetyltransferases: from drug metabolism and pharmacogenetics to drug discovery.British journal of pharmacology · 2014Review
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Azoreductases are involved in the bioremediation by bacteria of azo dyes found in waste water. In the gut flora, they activate azo pro-drugs, which are used for treatment of inflammatory bowel disease, releasing the active component 5-aminosalycilic acid. The bacterium P. aeruginosa has three azoreductase genes, paAzoR1, paAzoR2 and paAzoR3, which as recombinant enzymes have been shown to have different substrate specificities. The mechanism of azoreduction relies upon tautomerisation of the substrate to the hydrazone form. We report here the characterization of the P. aeruginosa azoreductase enzymes, including determining their thermostability, cofactor preference and kinetic constants against a range of their favoured substrates. The expression levels of these enzymes during growth of P. aeruginosa are altered by the presence of azo substrates. It is shown that enzymes that were originally described as azoreductases, are likely to act as NADH quinone oxidoreductases. The low sequence identities observed among NAD(P)H quinone oxidoreductase and azoreductase enzymes suggests convergent evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.