ArticlePloS one2010
Genome-wide analysis reveals loci encoding anti-macrophage factors in the human pathogen Burkholderia pseudomallei K96243.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Genetic diversity and transmission patterns ofMicrobial genomics · 2021Article
- Functional redundancy of Burkholderia pseudomallei phospholipase C enzymes and their role in virulence.Scientific reports · 2020Article
- An Evolutionary Arms Race BetweenFrontiers in microbiology · 2020Review
- Pediatric melioidosis in Sarawak, Malaysia: Epidemiological, clinical and microbiological characteristics.PLoS neglected tropical diseases · 2017Article
- Characterization of in vitro phenotypes of Burkholderia pseudomallei and Burkholderia mallei strains potentially associated with persistent infection in mice.Archives of microbiology · 2017Article
- Article
- Article
- Melioidosis: molecular aspects of pathogenesis.Expert review of anti-infective therapy · 2014Review
- Transcriptional profiles of Burkholderia pseudomallei reveal the direct and indirect roles of Sigma E under oxidative stress conditions.BMC genomics · 2014Article
- Delineating the importance of serum opsonins and the bacterial capsule in affecting the uptake and killing of Burkholderia pseudomallei by murine neutrophils and macrophages.PLoS neglected tropical diseases · 2014Article
- Variable virulence factors in Burkholderia pseudomallei (melioidosis) associated with human disease.PloS one · 2014Article
- Functional characterization of Burkholderia pseudomallei trimeric autotransporters.Infection and immunity · 2013Article
- Within-host evolution of Burkholderia pseudomallei over a twelve-year chronic carriage infection.mBio · 2013Article
- Burkholderia pseudomallei transcriptional adaptation in macrophages.BMC genomics · 2012Article
- Novel gain of function approaches for vaccine candidate identification in Burkholderia pseudomallei.Frontiers in cellular and infection microbiology · 2012Review
- Members of the salivary gland surface protein (SGS) family are major immunogenic components of mosquito saliva.The Journal of biological chemistry · 2011Article
- Autotransporters and Their Role in the Virulence of Burkholderia pseudomallei and Burkholderia mallei.Frontiers in microbiology · 2011Article
- Strategies for Intracellular Survival of Burkholderia pseudomallei.Frontiers in microbiology · 2011Article
- Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Burkholderia pseudomallei is an important human pathogen whose infection biology is still poorly understood. The bacterium is endemic to tropical regions, including South East Asia and Northern Australia, where it causes melioidosis, a serious disease associated with both high mortality and antibiotic resistance. B. pseudomallei is a Gram-negative facultative intracellular pathogen that is able to replicate in macrophages. However despite the critical nature of its interaction with macrophages, few anti-macrophage factors have been characterized to date. Here we perform a genome-wide gain of function screen of B. pseudomallei strain K96243 to identify loci encoding factors with anti-macrophage activity. We identify a total of 113 such loci scattered across both chromosomes, with positive gene clusters encoding transporters and secretion systems, enzymes/toxins, secondary metabolite, biofilm, adhesion and signal response related factors. Further phenotypic analysis of four of these regions shows that the encoded factors cause striking cellular phenotypes relevant to infection biology, including apoptosis, formation of actin 'tails' and multi-nucleation within treated macrophages. The detailed analysis of the remaining host of loci will facilitate genetic dissection of the interaction of this important pathogen with host macrophages and thus further elucidate this critical part of its infection cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.