ArticlePloS one2010
No evidence for XMRV in German CFS and MS patients with fatigue despite the ability of the virus to infect human blood cells in vitro.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 55 citations in OpenAlex.
- [Systems thinking, subjective findings and diagnostic "pigeonholing" in ME/CFS: A mainly qualitative public health study from a patient perspective].Deutsche medizinische Wochenschrift (1946) · 2024Article
- Article
- Chronic viral infections in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS).Journal of translational medicine · 2018Review
- Xenotropic Murine Leukemia Virus-Related Virus (XMRV) and the Safety of the Blood Supply.Clinical microbiology reviews · 2016Review
- Article
- Deficient EBV-specific B- and T-cell response in patients with chronic fatigue syndrome.PloS one · 2014Article
- No detection of the retrovirus xenotropic murine leukemia virus-related virus in individuals with hemophilia.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2013Article
- Emerging infectious agents and the nation's blood supply: responding to potential threats in the 21st century.Transfusion · 2013Article
- Utilization of replication-competent XMRV reporter-viruses reveals severe viral restriction in primary human cells.PloS one · 2013Article
- Fatigue and sleep disorders in multiple sclerosis: is obstructive sleep apnea a link?Sleep & breathing = Schlaf & Atmung · 2012Article
- Phylogenetic and biological analysis of a laboratory-generated gammaretrovirus xenotropic murine leukemia virus-related virus (XMRV).Virus genes · 2012Article
- No evidence for xenotropic murine leukemia-related virus infection in Sweden using internally controlled multiepitope suspension array serology.Clinical and vaccine immunology : CVI · 2012Article
- Recombinant origin, contamination, and de-discovery of XMRV.Current opinion in virology · 2012Review
- No evidence of XMRV infection in immunocompromised patients and HIV-positive individuals from Germany.Infection · 2012Article
- Intrinsic DNA synthesis fidelity of xenotropic murine leukemia virus-related virus reverse transcriptase.The FEBS journal · 2012Article
- Restricted replication of xenotropic murine leukemia virus-related virus in pigtailed macaques.Journal of virology · 2012Article
- Multiple sources of contamination in samples from patients reported to have XMRV infection.PloS one · 2012Article
- Characterization, mapping, and distribution of the two XMRV parental proviruses.Journal of virology · 2012Article
- No evidence for infection of UK prostate cancer patients with XMRV, BK virus, Trichomonas vaginalis or human papilloma viruses.PloS one · 2012Article
- XMRV induces cell migration, cytokine expression and tumor angiogenesis: are 22Rv1 cells a suitable prostate cancer model?PloS one · 2012Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundXenotropic murine leukemia virus-related virus (XMRV), a novel human retrovirus originally identified in prostate cancer tissues, has recently been associated with chronic fatigue syndrome (CFS), a disabling disease of unknown etiology affecting millions of people worldwide. However, several subsequent studies failed to detect the virus in patients suffering from these illnesses or in healthy subjects. Here we report the results of efforts to detect antibody responses and viral sequences in samples from a cohort of German CFS and relapsing remitting multiple sclerosis (MS) patients with fatigue symptoms. METHODOLOGY: Blood samples were taken from a cohort of 39 patients fulfilling the Fukuda/CDC criteria (CFS), from 112 patients with an established MS diagnosis and from 40 healthy donors. Fatigue severity in MS patients was assessed using the Fatigue Severity Scale (FSS). Validated Gag- and Env-ELISA assays were used to screen sera for XMRV antibodies. PHA-activated PBMC were cultured for seven days in the presence of IL-2 and DNA isolated from these cultures as well as from co-cultures of PBMC and highly permissive LNCaP cells was analyzed by nested PCR for the presence of the XMRV gag gene. In addition, PBMC cultures were exposed to 22Rv1-derived XMRV to assess infectivity and virus production.
conclusionNone of the screened sera from CFS and MS patients or healthy blood donors tested positive for XMRV specific antibodies and all PBMC (and PBMC plus LNCaP) cultures remained negative for XMRV sequences by nested PCR. These results argue against an association between XMRV infection and CFS and MS in Germany. However, we could confirm that PBMC cultures from healthy donors and from CFS patients can be experimentally infected by XMRV, resulting in the release of low levels of transmittable virus.
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