ArticlePLoS genetics2010
Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 75 citations in OpenAlex.
- Phosphorothioate DNA modification by BREX type 4 systems in the human gut microbiome.Nature communications · 2026Article
- Structural and mechanistic insights into the sequential dsDNA cleavage by SpCas12f1.Nucleic acids research · 2025Article
- Molecular Insight into the Recognition of DNA by the DndCDE Complex in DNA Phosphorothioation.International journal of molecular sciences · 2025Article
- Advances in Diversity, Evolutionary Dynamics and Biotechnological Potential of Restriction-Modification Systems.Microorganisms · 2025Review
- Nucleic acid recognition during prokaryotic immunity.Molecular cell · 2025Review
- Characterization of winged helix domain fusion endonucleases as N6-methyladenine-dependent type IV restriction systems.Frontiers in microbiology · 2024Article
- Characterization of a promiscuous DNA sulfur binding domain and application in site-directed RNA base editing.Nucleic acids research · 2023Article
- Base-excision restriction enzymes: expanding the world of epigenetic immune systems.DNA research : an international journal for rapid publication of reports on genes and genomes · 2023Article
- A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain.Synthetic and systems biotechnology · 2023Article
- Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE.Nature communications · 2022Article
- A Simple Evolutionary Model of Genetic Robustness After Gene Duplication.Journal of molecular evolution · 2022Article
- High-throughput sequencing of EcoWI restriction fragments maps the genome-wide landscape of phosphorothioate modification at base resolution.PLoS genetics · 2022Article
- System-Wide Analysis of the GATC-Binding Nucleoid-Associated Protein Gbn and Its Impact onmSystems · 2022Article
- The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes.Nature communications · 2021Article
- DNA backbone interactions impact the sequence specificity of DNA sulfur-binding domains: revelations from structural analyses.Nucleic acids research · 2020Article
- Protein Domain Guided Screen for Sequence Specific and Phosphorothioate-Dependent Restriction Endonucleases.Frontiers in microbiology · 2020Article
- Crystal structure of the EcoKMcrA N-terminal domain (NEco): recognition of modified cytosine bases without flipping.Nucleic acids research · 2019Article
- Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction-modification systems.Journal of industrial microbiology & biotechnology · 2019Article
- DNA phosphorothioate modification-a new multi-functional epigenetic system in bacteria.FEMS microbiology reviews · 2019Review
- Natural Products Containing 'Rare' Organophosphorus Functional Groups.Molecules (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many taxonomically diverse prokaryotes enzymatically modify their DNA by replacing a non-bridging oxygen with a sulfur atom at specific sequences. The biological implications of this DNA S-modification (phosphorothioation) were unknown. We observed that simultaneous expression of the dndA-E gene cluster from Streptomyces lividans 66, which is responsible for the DNA S-modification, and the putative Streptomyces coelicolor A(3)2 Type IV methyl-dependent restriction endonuclease ScoA3McrA (Sco4631) leads to cell death in the same host. A His-tagged derivative of ScoA3McrA cleaved S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Double-strand cleavage occurred 16-28 nucleotides away from the phosphorothioate links. DNase I footprinting demonstrated binding of ScoA3McrA to the Dcm methylation site, but no clear binding could be detected at the S-modified site under cleavage conditions. This is the first report of in vitro endonuclease activity of a McrA homologue and also the first demonstration of an enzyme that specifically cleaves S-modified DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.