ArticlePloS one2010
A novel technique for the in vivo imaging of autoimmune diabetes development in the pancreas by two-photon microscopy.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Autoimmunity in motion: Mechanisms of immune regulation and destruction revealed by in vivo imaging.Immunological reviews · 2022Review
- State-of-the-art microscopy to understand islets of Langerhans: what to expect next?Immunology and cell biology · 2021Review
- Live Intravital Intestine with Blood Flow Visualization in Neonatal Mice Using Two-photon Laser Scanning Microscopy.Bio-protocol · 2021Article
- Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose.Scientific reports · 2021Article
- Article
- Interference with pancreatic sympathetic signaling halts the onset of diabetes in mice.Science advances · 2020Article
- In vivo imaging of type 1 diabetes immunopathology using eye-transplanted islets in NOD mice.Diabetologia · 2019Article
- Two-photon intravital imaging of leukocyte migration during inflammation in the respiratory system.Acute and critical care · 2019Review
- Kinetics of corneal leukocytes by intravital multiphoton microscopy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Non-Invasive Multiphoton Imaging of Islets Transplanted Into the Pinna of the NOD Mouse Ear Reveals the Immediate Effect of Anti-CD3 Treatment in Autoimmune Diabetes.Frontiers in immunology · 2018Article
- Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas.Scientific reports · 2017Article
- Deep insights: intravital imaging with two-photon microscopy.Pflugers Archiv : European journal of physiology · 2016Review
- Live cell imaging to understand monocyte, macrophage, and dendritic cell function in atherosclerosis.The Journal of experimental medicine · 2016Review
- Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging.Diabetologia · 2016Article
- Dual-Reporter β-Cell-Specific Male Transgenic Rats for the Analysis of β-Cell Functional Mass and Enrichment by Flow Cytometry.Endocrinology · 2016Article
- A Deeper Look into Type 1 Diabetes - Imaging Immune Responses during Onset of Disease.Frontiers in immunology · 2016Review
- Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers.Scientific reports · 2015Article
- Dendritic cell subsets in type 1 diabetes: friend or foe?Frontiers in immunology · 2013Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type 1 diabetes (T1D) is characterized by the immune-mediated destruction of beta cells in the pancreas. Little is known about the in vivo dynamic interactions between T cells and beta cells or the kinetic behavior of other immune cell subsets in the pancreatic islets. Utilizing multiphoton microscopy we have designed a technique that allows for the real-time visualization of diabetogenic T cells and dendritic cells in pancreatic islets in a live animal, including their interplay with beta cells and the vasculature. Using a custom designed stage, the pancreas was surgically exposed under live conditions so that imaging of islets under intact blood pressure and oxygen supply became possible. We demonstrate here that this approach allows for the tracking of diabetogenic leukocytes as well as vascularization phenotype of islets and accumulation of dendritic cells in islets during diabetes pathogenesis. This technique should be useful in mapping crucial kinetic events in T1D pathogenesis and in testing the impact of immune based interventions on T cell migration, extravasation and islet destruction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.