ArticlePLoS computational biology2010
Kinetics of rhodopsin deactivation and its role in regulating recovery and reproducibility of rod photoresponse.
Article in PLoS computational biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The effects of bicarbonate on the aberrant photon response in murine rod photoreceptors.Biophysical journal · 2026Article
- Analysis of dim-light responses in rod and cone photoreceptors with altered calcium kinetics.Journal of mathematical biology · 2023Article
- Photoreceptor physiology and evolution: cellular and molecular basis of rod and cone phototransduction.The Journal of physiology · 2022Review
- Effects of cell size and bicarbonate on single photon response variability in retinal rods.Frontiers in molecular neuroscience · 2022Article
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- Multi-scale, numerical modeling of spatio-temporal signaling in cone phototransduction.PloS one · 2019Article
- Local, nonlinear effects of cGMP and Ca2+ reduce single photon response variability in retinal rods.PloS one · 2019Article
- Quantitative modeling of the molecular steps underlying shut-off of rhodopsin activity in rod phototransduction.Molecular vision · 2016Article
- The phototransduction machinery in the rod outer segment has a strong efficacy gradient.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- cGMP in mouse rods: the spatiotemporal dynamics underlying single photon responses.Frontiers in molecular neuroscience · 2015Review
- Engineering visual arrestin-1 with special functional characteristics.The Journal of biological chemistry · 2013Article
- Calcium feedback to cGMP synthesis strongly attenuates single-photon responses driven by long rhodopsin lifetimes.Neuron · 2012Article
- Speed, sensitivity, and stability of the light response in rod and cone photoreceptors: facts and models.Progress in retinal and eye research · 2012Review
- Rhodopsin expression level affects rod outer segment morphology and photoresponse kinetics.PloS one · 2012Article
- The functional cycle of visual arrestins in photoreceptor cells.Progress in retinal and eye research · 2011Review
- Identification of key factors that reduce the variability of the single photon response.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Robust self-association is a common feature of mammalian visual arrestin-1.Biochemistry · 2011Article
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Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The single photon response (SPR) in vertebrate phototransduction is regulated by the dynamics of R* during its lifetime, including the random number of phosphorylations, the catalytic activity and the random sojourn time at each phosphorylation level. Because of this randomness the electrical responses are expected to be inherently variable. However the SPR is highly reproducible. The mechanisms that confer to the SPR such a low variability are not completely understood. The kinetics of rhodopsin deactivation is investigated by a Continuous Time Markov Chain (CTMC) based on the biochemistry of rhodopsin activation and deactivation, interfaced with a spatio-temporal model of phototransduction. The model parameters are extracted from the photoresponse data of both wild type and mutant mice, having variable numbers of phosphorylation sites and, with the same set of parameters, the model reproduces both WT and mutant responses. The sources of variability are dissected into its components, by asking whether a random number of turnoff steps, a random sojourn time between steps, or both, give rise to the known variability. The model shows that only the randomness of the sojourn times in each of the phosphorylated states contributes to the Coefficient of Variation (CV) of the response, whereas the randomness of the number of R* turnoff steps has a negligible effect. These results counter the view that the larger the number of decay steps of R*, the more stable the photoresponse is. Our results indicate that R* shutoff is responsible for the variability of the photoresponse, while the diffusion of the second messengers acts as a variability suppressor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.