ArticleThe EMBO journal1990
A novel upstream element compensates for an ineffectual octamer motif in an immunoglobulin V kappa promoter.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Mouse lymphoid cell line selected to have high immunoglobulin promoter activity.Molecular and cellular biology · 2002Article
- Transcription factor AP-4 is a ligand for immunoglobulin-kappa promoter E-box elements.The Biochemical journal · 2001Article
- Efficient transcription of an immunoglobulin kappa promoter requires specific sequence elements overlapping with and downstream of the transcriptional start site.Nucleic acids research · 1997Article
- Octamer independent activation of transcription from the kappa immunoglobulin germline promoter.Nucleic acids research · 1996Article
- A defective Vkappa A2 allele in Navajos which may play a role in increased susceptibility to haemophilus influenzae type b disease.The Journal of clinical investigation · 1996Article
- CCAAT box binding protein NF-Y facilitates in vivo recruitment of upstream DNA binding transcription factors.The EMBO journal · 1994Article
- Analysis of the imperfect octamer-containing human immunoglobulin VH6 gene promoter.Nucleic acids research · 1994Article
- Bidirectional transcription from the human immunoglobulin VH6 gene promoter.Nucleic acids research · 1994Article
- Kappa immunoglobulin promoters and enhancers display developmentally controlled interactions.Nucleic acids research · 1993Article
- In vitro and in vivo binding of human immunodeficiency virus type 1 Tat protein and Sp1 transcription factor.Journal of virology · 1993Article
- A mammalian DNA-binding protein that contains a chromodomain and an SNF2/SWI2-like helicase domain.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Cloning of human and bovine homologs of SNF2/SWI2: a global activator of transcription in yeast S. cerevisiae.Nucleic acids research · 1992Article
- PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity.Molecular and cellular biology · 1992Article
- Two conserved essential motifs of the murine immunoglobulin lambda enhancers bind B-cell-specific factors.Molecular and cellular biology · 1992Article
- Isolation of a candidate repressor/activator, NF-E1 (YY-1, delta), that binds to the immunoglobulin kappa 3' enhancer and the immunoglobulin heavy-chain mu E1 site.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Functional modularity in the SP6 kappa promoter.Nucleic acids research · 1991Article
- The genomic organization of immunoglobulin VH genes in Xenopus laevis shows evidence for interspersion of families.Nucleic acids research · 1991Article
- Promoters with the octamer DNA motif (ATGCAAAT) can be ubiquitous or cell type-specific depending on binding affinity of the octamer site and Oct-factor concentration.Nucleic acids research · 1991Article
- Two closely related kappa variable region pseudogenes pose an evolutionary paradox.Immunogenetics · 1991Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The octamer (or dc/cd) motif is considered to be a critical component of all immunoglobulin (Ig) promoters. Although the sequence of this motif is highly conserved among most Ig promoters, there are some notable examples in which efficiently expressed Ig genes contain divergent octamers with base substitutions that are demonstrably deleterious when tested with heterologous proximal promoter elements. To elucidate the mechanisms that enable these naturally occurring Ig genes to cope with divergent octamers, we analyzed two such promoters with regard to their ability to interact with relevant transcription factors. We found that the divergent octamer in the kappa O germline promoter strongly binds both Oct-1 and Oct-2 factors, presumably because of compensatory contributions by flanking DNA sequences. A more surprising result was obtained with the V kappa 19 promoter. In this case, the divergent octamer is a very weak Oct factor binding site and, without help from another upstream element, is inadequate for efficient promoter function. This additional element, termed kappa Y because of its high pyrimidine content (CTTCCTTA), serves as a binding site for a novel lymphoid-specific factor. When the divergent V kappa 19 octamer was converted to a strong Oct factor binding site by a single point mutation, the need for kappa Y was obviated. Interestingly, VH promoters that contain the same divergent octamer also contain an upstream element that is very similar to kappa Y.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.