ArticleMolecular plant pathology2011
Functional mapping of harpin HrpZ of Pseudomonas syringae reveals the sites responsible for protein oligomerization, lipid interactions and plant defence induction.
Article in Molecular plant pathology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 71 citations in OpenAlex.
- Role of Plasmodesmata During Plant-Pathogen Interactions.The plant pathology journal · 2026Review
- The engineered Immunoprotein enhance broad-spectrum disease resistance in solanaceous crops.BMC plant biology · 2026Article
- Overexpression ofPlant signaling & behavior · 2025Article
- Review
- HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon.Molecular plant pathology · 2025Article
- Uncovering the Interaction Interface Between Harpin (Hpa1) and Rice Aquaporin (OsPIP1;3) Through Protein-Protein Docking: An In Silico Approach.Molecular biotechnology · 2024Article
- Pseudomonas syringae Type III Secretion Protein HrpP Manipulates Plant Immunity To Promote Infection.Microbiology spectrum · 2023Article
- Exploring structural features and potential lipid interactions of Pseudomonas aeruginosa type three secretion effector PemB by spectroscopic and calorimetric experiments.Protein science : a publication of the Protein Society · 2023Article
- Characteristics, Roles and Applications of Proteinaceous Elicitors from Pathogens in Plant Immunity.Life (Basel, Switzerland) · 2023Review
- The stability of the coiled-coil structure near to N-terminus influence the heat resistance of harpin proteins from Xanthomonas.BMC microbiology · 2020Article
- Genomic Characterisation of Mushroom Pathogenic Pseudomonads and Their Interaction with Bacteriophages.Viruses · 2020Article
- Contributions and Limitations of Biophysical Approaches to Study of the Interactions between Amphiphilic Molecules and the Plant Plasma Membrane.Plants (Basel, Switzerland) · 2020Review
- HpaXpm, a novel harpin of Xanthomonas phaseoli pv. manihotis, acts as an elicitor with high thermal stability, reduces disease, and promotes plant growth.BMC microbiology · 2020Article
- Three Proteins (Hpa2, HrpF and XopN) Are Concomitant Type III Translocators in Bacterial Blight Pathogen of Rice.Frontiers in microbiology · 2020Article
- The Type III Accessory Protein HrpE ofMicroorganisms · 2019Article
- Real-time monitoring of translocation of selected type-III effectors fromJournal of biosciences · 2019Article
- Rice aquaporin PIP1;3 and harpin Hpa1 of bacterial blight pathogen cooperate in a type III effector translocation.Journal of experimental botany · 2019Article
- Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies.International journal of molecular sciences · 2019Article
- Plant Aquaporins in Infection by and Immunity Against Pathogens - A Critical Review.Frontiers in plant science · 2019Article
- Hpa1 is a type III translocator in Xanthomonas oryzae pv. oryzae.BMC microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Harpin HrpZ is one of the most abundant proteins secreted through the pathogenesis-associated type III secretion system of the plant pathogen Pseudomonas syringae. HrpZ shows membrane-binding and pore-forming activities in vitro, suggesting that it could be targeted to the host cell plasma membrane. We studied the native molecular forms of HrpZ and found that it forms dimers and higher order oligomers. Lipid binding by HrpZ was tested with 15 different membrane lipids, with HrpZ interacting only with phosphatidic acid. Pore formation by HrpZ in artificial lipid vesicles was found to be dependent on the presence of phosphatidic acid. In addition, HrpZ was able to form pores in vesicles prepared from Arabidopsis thaliana plasma membrane, providing evidence for the suggested target of HrpZ in the host. To map the functions associated with HrpZ, we constructed a comprehensive series of deletions in the hrpZ gene derived from P. syringae pv. phaseolicola, and studied the mutant proteins. We found that oligomerization is mainly mediated by a region near the C-terminus of the protein, and that the same region is also essential for membrane pore formation. Phosphatidic acid binding seems to be mediated by two regions separate in the primary structure. Tobacco, a nonhost plant, recognizes, as a defence elicitor, a 24-amino-acid HrpZ fragment which resides in the region indispensable for the oligomerization and pore formation functions of HrpZ.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.