ArticleJournal of cellular and molecular medicine2011
Endothelial progenitor cells are integrated in newly formed capillaries and alter adjacent fibrovascular tissue after subcutaneous implantation in a fibrin matrix.
Article in Journal of cellular and molecular medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
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- The regenerative journey: exploring stem cell roles from injury detection to tissue repair.Stem cell research & therapy · 2025Review
- Gap Junctional Interaction of Endothelial Progenitor Cells (EPC) with Endothelial Cells Induces Angiogenic Network Formation In Vitro.International journal of molecular sciences · 2025Article
- In Vivo Vascularization of Cell-Supplemented Spider Silk-Based Hydrogels in the Arteriovenous Loop Model.Biomimetics (Basel, Switzerland) · 2025Article
- The pro-angiogenic role of hypoxia inducible factor stabilizer FG-4592 and its application in an in vivo tissue engineering chamber model.Scientific reports · 2019Article
- Prevascularized mesenchymal stem cell-sheets increase survival of random skin flaps in a nude mouse model.American journal of translational research · 2019Article
- Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material.BioMed research international · 2017Article
- Vessel transformation in chronic wounds under topical negative pressure therapy: an immunohistochemical analysis.International wound journal · 2015Article
- Management of chronic osteomyelitis of the tibia with life-threatening complications under negative pressure wound therapy and isolation of Helcococcus kunzii.International wound journal · 2015Article
- PHDs inhibitor DMOG promotes the vascularization process in the AV loop by HIF-1a up-regulation and the preliminary discussion on its kinetics in rat.BMC biotechnology · 2014Article
- A hundred patients with vertical rectus abdominis myocutaneous (VRAM) flap for pelvic reconstruction after total pelvic exenteration.International journal of colorectal disease · 2014Article
- A novel early precursor cell population from rat bone marrow promotes angiogenesis in vitro.BMC cell biology · 2014Article
- Development of a new pre-vascularized tissue-engineered construct using pre-differentiated rADSCs, arteriovenous vascular bundle and porous nano-hydroxyapatide-polyamide 66 scaffold.BMC musculoskeletal disorders · 2013Article
- [Osteomyelitis: treatment concepts from the plastic surgeon's point of view].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2013Review
- Cancer research by means of tissue engineering--is there a rationale?Journal of cellular and molecular medicine · 2013Review
- Evaluation of angiogenesis of bioactive glass in the arteriovenous loop model.Tissue engineering. Part C, Methods · 2013Article
- Adipose-derived stem cells promote angiogenesis and tissue formation for in vivo tissue engineering.Tissue engineering. Part A · 2013Article
- Myogenic differentiation of mesenchymal stem cells in a newly developed neurotised AV-loop model.BioMed research international · 2013Article
- [Pathogenesis, classification and diagnosis of necrotizing soft tissue infections].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2012Review
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Authors and funding
11 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vascularization of bioartificial matrices is crucial for successful tissue engineering. Endothelial progenitor cells (EPC) have shown vascularization potential in ischemic conditions and may also support blood vessel formation in tissue-engineered matrices. The aim of our study was to investigate the impact of a well-characterized murine embryonal EPC line (T17b-EPC) on vascularization and fibrovascular granulation tissue formation after suspension in a fibrine matrix followed by subcutaneous implantation in a separation chamber in rats. EPC were fluorescently labelled in vitro prior to implantation. After 3, 7 or 14 days, animals were killed followed by explantation and histological analysis of the constructs. Before the end of the experiment, Bandeirea Simplicifolia lectin was intravenously injected to mark the vascular ingrowth into the implanted constructs. The transplanted cells were histologically detected at all time-points and located almost exclusively within the fibrin matrix at day 3 but the number of cells in the clot continuously decreased over day 7 to day 14. Conversely, cells were detected within the newly formed granulation tissue in increasing numbers from day 3 over day 7 to day 14. Transplanted cells were also found in the intermuscular septa. Cell viability was confirmed by use of an EPC clone expressing β-galactosidase. Fluorescence microscopy demonstrated integration of the transplanted cells in newly formed blood vessels within the fibrovascular granulation tissue adjacent to the fibrin clot. Presence of cells in the fibrin clot lead to thicker granulation tissue and an increased blood vessel diameter compared to cell-free controls. Organ standard controls showed presence of the transplanted cells in spleens at day 14 after transplantation. In summary, EPC exhibited biological activity after subcutaneous implantation in a fibrin matrix by migration from the fibrin clot into the granulation tissue and along intermuscular septae, undergoing differentiation into mature endothelial cells and integration into newly formed blood vessels and altering fibrovascular granulation tissue development. EPC may hold promise to modulate blood vessel formation in bioartificial matrices.
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