ArticleCell proliferation2011
In vitro mesenchymal stem cell differentiation after mechanical stimulation.
Article in Cell proliferation, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 66 citations in OpenAlex.
- Non-coding RNAs mediate mechanical load-regulating bone metabolic homeostasis.Frontiers in cell and developmental biology · 2025Review
- Stem Cells: Present Understanding and Prospects for Regenerative Dentistry.Journal of functional biomaterials · 2024Review
- Integrin αVβ3 antagonist-c(RGDyk) peptide attenuates the progression of ossification of the posterior longitudinal ligament by inhibiting osteogenesis and angiogenesis.Molecular medicine (Cambridge, Mass.) · 2024Article
- Knowledge mapping and research trends of stem cell in wound healing: A bibliometric analysis.International wound journal · 2024Article
- Article
- HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCs.Scientific reports · 2023Article
- Biomechanical Modulation of Dental Pulp Stem Cell (DPSC) Properties for Soft Tissue Engineering.Bioengineering (Basel, Switzerland) · 2023Article
- Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head.Frontiers in cell and developmental biology · 2023Article
- Low-intensity ultrasound combined with allogenic adipose-derived mesenchymal stem cells (AdMSCs) in radiation-induced skin injury treatment.Scientific reports · 2020Article
- Dysfunctional stem and progenitor cells impair fracture healing with age.World journal of stem cells · 2019Review
- Effects of Cyclic Tensile Strain on Oxidative Stress and the Function of Schwann Cells.BioMed research international · 2018Article
- Low-intensity pulsed ultrasound upregulates pro-myelination indicators of Schwann cells enhanced by co-culture with adipose-derived stem cells.Cell proliferation · 2016Article
- Effects of uniaxial cyclic stretch loading on morphology of adipose derived stem cells.Tissue engineering and regenerative medicine · 2016Article
- Mechanical loading inhibits hypertrophy in chondrogenically differentiating hMSCs within a biomimetic hydrogel.Journal of materials chemistry. B · 2016Article
- Vibration stimuli and the differentiation of musculoskeletal progenitor cells: Review of results in vitro and in vivo.World journal of stem cells · 2015Review
- Physicochemical and biomechanical stimuli in cell-based articular cartilage repair.Current rheumatology reports · 2015Review
- Cyclic mechanical stress modulates neurotrophic and myelinating gene expression of Schwann cells.Cell proliferation · 2015Article
- Gene expression responses to mechanical stimulation of mesenchymal stem cells seeded on calcium phosphate cement.Tissue engineering. Part A · 2013Article
- Influence of discrete and continuous culture conditions on human mesenchymal stem cell lineage choice in RGD concentration gradient hydrogels.Biomacromolecules · 2013Article
- Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice.Chinese journal of integrative medicine · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesMesenchymal stem cells (MSC) are multipotent cells capable of differentiating into adipocytic, chondrocytic and osteocytic lineages on suitable stimulation. We have hypothesized that mechanical loading may influence MSC differentiation and alter their phenotype accordingly. MATERIALS AND
methodsMouse bone marrow-derived MSC were established in vitro by differential adherence to plastic culture plates and grown in low glucose medium with 10% foetal calf serum and growth factors. Cells grew out and were subcultured up to 20 times. Differentiation protocols were followed for several cell lineages. Clones with trilineage potential were seeded in type I collagen gels and incubated in a tensioning force bioreactor and real-time cell-derived forces were recorded. Gels were fixed and sectioned for light and electron microscopy.
resultsCell monolayers of parent and cloned mouse bone marrow-derived MSC differentiated into adipocytes, osteocytes and chondrocytes, but not into cardiomyocytes, myotubes or neuronal cells. When cast into type I collagen gels and placed in tensioning bioreactors, MSC differentiated into fibroblast-like cells typical of tissue stroma, and upregulated α-smooth muscle actin, but rarely upregulated desmin. Electron microscopy showed collagen and elastin fibre synthesis into the matrix.
conclusionsThese experiments confirmed that MSC cell fate choice depends on minute, cell-derived forces. Applied force could assist in commercial manufacture of cultured bio-engineered prostheses for regenerative medicine as it mimics tissue stresses and constitutes a good model for development of tissue substitutes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.