ArticleJournal of biomedical optics
Identification of different bacterial species in biofilms using confocal Raman microscopy.
Article in Journal of biomedical optics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Article
- AC Electromagnetic Field Controls the Biofilms on the Glass Surface byMaterials (Basel, Switzerland) · 2023Article
- Identification of antibiotic resistance and virulence-encoding factors in Klebsiella pneumoniae by Raman spectroscopy and deep learning.Microbial biotechnology · 2022Article
- Mapping of a Subgingival Dual-Species Biofilm Model Using Confocal Raman Microscopy.Frontiers in microbiology · 2021Article
- Article
- Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues.Journal of biophotonics · 2019Article
- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
- Optical Sensing of Microbial Life on Surfaces.Applied and environmental microbiology · 2015Review
- Tackling the minority: sulfate-reducing bacteria in an archaea-dominated subsurface biofilm.The ISME journal · 2013Article
- High-speed hyperspectral Raman imaging for label-free compositional microanalysis.Biomedical optics express · 2013Article
- Raman microspectroscopy for species identification and mapping within bacterial biofilms.AMB Express · 2012Article
- Raman spectroscopy detects deterioration in biomechanical properties of bone in a glucocorticoid-treated mouse model of rheumatoid arthritis.Journal of biomedical optics · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Confocal Raman microspectroscopy is used to discriminate between different species of bacteria grown in biofilms. Tests are performed using two bacterial species, Streptococcus sanguinis and Streptococcus mutans, which are major components of oral plaque and of particular interest due to their association with healthy and cariogenic plaque, respectively. Dehydrated biofilms of these species are studied as a simplified model of dental plaque. A prediction model based on principal component analysis and logistic regression is calibrated using pure biofilms of each species and validated on pure biofilms grown months later, achieving 96% accuracy in prospective classification. When biofilms of the two species are partially mixed together, Raman-based identifications are achieved within ∼2 μm of the boundaries between species with 97% accuracy. This combination of spatial resolution and predication accuracy should be suitable for forming images of species distributions within intact two-species biofilms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.