ArticleJournal of biomedicine & biotechnology2011
Construction and characterization of an infectious murine gammaherpesivrus-68 bacterial artificial chromosome.
Article in Journal of biomedicine & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Liquid-liquid phase separation mediates the formation of herpesvirus assembly compartments.The Journal of cell biology · 2023Article
- Functional Identification and Characterization of the Nuclear Egress Complex of a Gammaherpesvirus.Journal of virology · 2019Article
- Proteomics of Bronchoalveolar Lavage Fluid Reveals a Lung Oxidative Stress Response in Murine Herpesvirus-68 Infection.Viruses · 2018Article
- Rapid and efficient in vitro excision of BAC sequences from herpesvirus genomes using Cre-mediated recombination.Journal of virological methods · 2018Article
- A DNA-sensing-independent role of a nuclear RNA helicase, DHX9, in stimulation of NF-κB-mediated innate immunity against DNA virus infection.Nucleic acids research · 2018Article
- A Herpesvirus Protein Selectively Inhibits Cellular mRNA Nuclear Export.Cell host & microbe · 2016Article
- Murine Gammaherpesvirus 68 ORF48 Is an RTA-Responsive Gene Product and Functions in both Viral Lytic Replication and Latency during In Vivo Infection.Journal of virology · 2015Article
- Gammaherpesvirus Tegument Protein ORF33 Is Associated With Intranuclear Capsids at an Early Stage of the Tegumentation Process.Journal of virology · 2015Article
- Identification of the Essential Role of Viral Bcl-2 for Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication.Journal of virology · 2015Article
- A gammaherpesvirus establishes persistent infection in neuroblastoma cells.Molecules and cells · 2014Article
- ZAP inhibits murine gammaherpesvirus 68 ORF64 expression and is antagonized by RTA.Journal of virology · 2013Article
- Zinc finger antiviral protein inhibits murine gammaherpesvirus 68 M2 expression and regulates viral latency in cultured cells.Journal of virology · 2012Article
- Distinct domains in ORF52 tegument protein mediate essential functions in murine gammaherpesvirus 68 virion tegumentation and secondary envelopment.Journal of virology · 2012Article
- The anti-interferon activity of conserved viral dUTPase ORF54 is essential for an effective MHV-68 infection.PLoS pathogens · 2011Article
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Authors and funding
6 authors.
Funding
Abstract
Here we describe the cloning of a sequenced WUMS isolate of murine gammaherpesvirus-68 (MHV-68, γHV-68, also known as MuHV-4) as a bacterial artificial chromosome (BAC). We engineered the insertion of the BAC sequence flanked by loxP sites into the left end of the viral genome before the M1 open reading frame. The infectious viruses were reconstituted following transfection of the MHV-68 BAC DNA into cells. The MHV-68 BAC-derived virus replicated indistinguishably from the wild-type virus in cultured cells. Excision of the BAC insert was efficiently achieved by coexpressing the Cre recombinase. Although the BAC insertion did not significantly affect acute productive infection in the lung, it severely compromised the ability of MHV-68 to establish splenic latency. Removal of the BAC sequence restored the wild-type level of latency. Site-specific mutagenesis was carried out by RecA-mediated recombination to demonstrate that this infectious BAC clone can be used for genetic studies of MHV-68.
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