ArticleArchaea (Vancouver, B.C.)2010
A novel pathway for the biosynthesis of heme in Archaea: genome-based bioinformatic predictions and experimental evidence.
Article in Archaea (Vancouver, B.C.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 71 citations in OpenAlex.
- Milestones in the Elucidation of Heme Biosynthesis.Chemical reviews · 2026Review
- Function, Structure, and Regulation of Nitrogen Fixation-like Metalloproteins for Nitrogen, Energy, Carbon, and Sulfur Metabolism.Chemical reviews · 2026Review
- Heme in Bacterial Pathogenesis and as an Antimicrobial Target.Chemical reviews · 2025Review
- Transcriptomic profiling of haloarchaeal denitrification through RNA-Seq analysis.Applied and environmental microbiology · 2024Article
- The Radical SAM Heme Synthase AhbD fromBiomolecules · 2023Article
- Proteomic Analysis of Methanococcus voltae Grown in the Presence of Mineral and Nonmineral Sources of Iron and Sulfur.Microbiology spectrum · 2022Article
- Radical SAM Enzymes Involved in Tetrapyrrole Biosynthesis and Insertion.ACS bio & med chem Au · 2022Review
- Pathways of Iron and Sulfur Acquisition, Cofactor Assembly, Destination, and Storage in Diverse Archaeal Methanogens and Alkanotrophs.Journal of bacteriology · 2021Article
- Open Issues for Protein Function Assignment inGenes · 2021Article
- Enigmatic Evolutionary History of Porphobilinogen Deaminase in Eukaryotic Phototrophs.Biology · 2021Article
- A Structurally Novel Lipoyl Synthase in the Hyperthermophilic Archaeon Thermococcus kodakarensis.Applied and environmental microbiology · 2020Article
- TheInfection and immunity · 2020Article
- Biosynthesis of the modified tetrapyrroles-the pigments of life.The Journal of biological chemistry · 2020Review
- Systematic bacterialization of yeast genes identifies a near-universally swappable pathway.eLife · 2017Article
- Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.Microbiology and molecular biology reviews : MMBR · 2017Review
- Growth phenotype analysis of heme synthetic enzymes in a halophilic archaeon, Haloferax volcanii.PloS one · 2017Article
- The Plasma Membrane Protein Nce102 Implicated in Eisosome Formation Rescues a Heme Defect in Mitochondria.The Journal of biological chemistry · 2016Article
- Transcriptomes of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Exposed to Metal "Shock" Reveal Generic and Specific Metal Responses.Applied and environmental microbiology · 2016Article
- The auxiliary [4Fe-4S] cluster of the Radical SAM heme synthase fromChemical science · 2016Article
- Recent advances in the biosynthesis of modified tetrapyrroles: the discovery of an alternative pathway for the formation of heme and heme d 1.Cellular and molecular life sciences : CMLS · 2014Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Heme is an essential prosthetic group for many proteins involved in fundamental biological processes in all three domains of life. In Eukaryota and Bacteria heme is formed via a conserved and well-studied biosynthetic pathway. Surprisingly, in Archaea heme biosynthesis proceeds via an alternative route which is poorly understood. In order to formulate a working hypothesis for this novel pathway, we searched 59 completely sequenced archaeal genomes for the presence of gene clusters consisting of established heme biosynthetic genes and colocalized conserved candidate genes. Within the majority of archaeal genomes it was possible to identify such heme biosynthesis gene clusters. From this analysis we have been able to identify several novel heme biosynthesis genes that are restricted to archaea. Intriguingly, several of the encoded proteins display similarity to enzymes involved in heme d(1) biosynthesis. To initiate an experimental verification of our proposals two Methanosarcina barkeri proteins predicted to catalyze the initial steps of archaeal heme biosynthesis were recombinantly produced, purified, and their predicted enzymatic functions verified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.