ArticleBMC cell biology2010
The chromosomal association/dissociation of the chromatin insulator protein Cp190 of Drosophila melanogaster is mediated by the BTB/POZ domain and two acidic regions.
Article in BMC cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Identification of a Highly Conserved Region Critical for the Functionality of the Cp190 Protein in Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2025Article
- Article
- New Drosophila promoter-associated architectural protein Mzfp1 interacts with CP190 and is required for housekeeping gene expression and insulator activity.Nucleic acids research · 2024Article
- Development of a New Model System to Study Long-Distance Interactions Supported by Architectural Proteins.International journal of molecular sciences · 2024Article
- The N-Terminal Part ofInternational journal of molecular sciences · 2023Article
- The MADF-BESS Protein CP60 Is Recruited to Insulators via CP190 and Has Redundant Functions inInternational journal of molecular sciences · 2023Article
- Study of the in Vivo Functional Role of Mutations in the BTB Domain of the CP190 Protein of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2023Article
- Topological screen identifies hundreds of Cp190- and CTCF-dependentScience advances · 2023Article
- <i>Drosophila</i> insulator proteins exhibit in vivo liquid-liquid phase separation properties.Life science alliance · 2022Article
- Mechanisms of CP190 Interaction with Architectural Proteins in Drosophila Melanogaster.International journal of molecular sciences · 2021Article
- Dissecting protein domain variability in the core RNA interference machinery of five insect orders.RNA biology · 2021Article
- BACH1, the master regulator of oxidative stress, has a dual effect on CFTR expression.The Biochemical journal · 2021Article
- Mechanisms of Enhancer-Promoter Interactions in Higher Eukaryotes.International journal of molecular sciences · 2021Review
- HIPP1 stabilizes the interaction between CP190 and Su(Hw) in the Drosophila insulator complex.Scientific reports · 2019Article
- The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression.Scientific reports · 2019Article
- Functional dissection of the developmentally restricted BEN domain chromatin boundary factor Insensitive.Epigenetics & chromatin · 2019Article
- Interactions between BTB domain of CP190 and two adjacent regions in Su(Hw) are required for the insulator complex formation.Chromosoma · 2018Article
- Article
- Opbp is a new architectural/insulator protein required for ribosomal gene expression.Nucleic acids research · 2017Article
- Multiple interactions are involved in a highly specific association of the Mod(mdg4)-67.2 isoform with the Su(Hw) sites inOpen biology · 2017Article
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5 authors.
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Abstract
backgroundChromatin insulators or boundary elements are a class of functional elements in the eukaryotic genome. They regulate gene transcription by interfering with promoter-enhancer communication. The Cp190 protein of Drosophila melanogaster is essential to the function of at least three-types of chromatin insulator complexes organized by Su(Hw), CTCF and BEAF32.
resultsWe mapped functional regions of Cp190 in vivo and identified three domains that are essential for the insulator function and for the viability of flies: the BTB/POZ domain, an aspartic acid-rich (D-rich) region and a C-terminal glutamic acid-rich (E-rich) region. Other domains including the centrosomal targeting domain and the zinc fingers are dispensable. The N-terminal CP190BTB-D fragment containing the BTB/POZ domain and the D-rich region is sufficient to mediate association with all three types of insulator complexes. The fragment however is not sufficient for insulator activity or viability. The Cp190 and CP190BTB-D are regulated differently in cells treated with heat-shock. The Cp190 dissociated from chromosomes during heat-shock, indicating that dissociation of Cp190 with chromosomes can be regulated. In contrast, the CP190BTB-D fragment didn't dissociate from chromosomes in the same heat-shocked condition, suggesting that the deleted C-terminal regions have a role in regulating the dissociation of Cp190 with chromosomes.
conclusionsThe N-terminal fragment of Cp190 containing the BTB/POZ domain and the D-rich region mediates association of Cp190 with all three types of insulator complexes and that the E-rich region of Cp190 is required for dissociation of Cp190 from chromosomes during heat-shock. The heat-shock-induced dissociation is strong evidence indicating that dissociation of the essential insulator protein Cp190 from chromosomes is regulated. Our results provide a mechanism through which activities of an insulator can be modulated by internal and external cues.
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