ArticleApplied and environmental microbiology2011
Specific real-time PCR for simultaneous detection and identification of Legionella pneumophila serogroup 1 in water and clinical samples.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 111 citations in OpenAlex.
- Diagnostic Accuracy of PCR Alone and Compared to Urinary Antigen Testing for Detection of Legionella spp.: a Systematic Review.Journal of clinical microbiology · 2016Pooled it
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- A novel hybridization capture method for direct whole genome sequencing of clinical specimens to inform Legionnaires' disease investigations.Journal of clinical microbiology · 2024Article
- A rapid and reliable method for earlyFrontiers in microbiology · 2024Article
- A multi-center validation study on the discrimination ofFrontiers in microbiology · 2023Article
- Hot water plumbing in residences and office buildings have distinctive risk of Legionella pneumophila contamination.International journal of hygiene and environmental health · 2022Article
- Presence of Viable, Clinically RelevantMicrobiology spectrum · 2022Article
- Legionnaires' Disease: Update on Diagnosis and Treatment.Infectious diseases and therapy · 2022Review
- Non-IDCases · 2022Article
- Comparison of the culture method with multiplex PCR for the confirmation of Legionella spp. and Legionella pneumophila.Journal of applied microbiology · 2021Article
- Development of a Multiplex-PCR Serotyping Assay for Characterizing Legionella pneumophila Serogroups Based on the Diversity of Lipopolysaccharide Biosynthetic Loci.Journal of clinical microbiology · 2021Article
- Improvements to the Success of Outbreak Investigations of Legionnaires' Disease: 40 Years of Testing and Investigation in New York State.Applied and environmental microbiology · 2021Article
- Quantification of Legionella pneumophila by qPCR and culture in tap water with different concentrations of residual disinfectants and heterotrophic bacteria.The Science of the total environment · 2021Article
- Interactive Effects of Copper Pipe, Stagnation, Corrosion Control, and Disinfectant Residual Influenced Reduction ofPathogens (Basel, Switzerland) · 2020Article
- Legionnaires' Disease Cases at a Large Community Hospital-Common and Underdiagnosed.International journal of environmental research and public health · 2020Article
- The Impact of Storms onFrontiers in microbiology · 2020Article
- Impact of Chlorine and Chloramine on the Detection and Quantification of Legionella pneumophila andApplied and environmental microbiology · 2019Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 1 country.
Funding
Abstract
Legionella pneumophila, a bacterium that replicates within aquatic amoebae and persists in the environment as a free-living microbe, is the causative agent of Legionnaires' disease. Among the many Legionella species described, L. pneumophila is associated with 90% of human disease, and within the 15 serogroups (Sg), L. pneumophila Sg1 causes more than 84% of Legionnaires' disease worldwide. Thus, rapid and specific identification of L. pneumophila Sg1 is of the utmost importance for evaluation of the contamination of collective water systems and the risk posed. Previously we had shown that about 20 kb of the 33-kb locus carrying the genes coding for the proteins involved in lipopolysaccharide biosynthesis (LPS gene cluster) by L. pneumophila was highly specific for Sg1 strains and that three genes (lpp0831, wzm, and wzt) may serve as genetic markers. Here we report the sequencing and comparative analyses of this specific region of the LPS gene cluster in L. pneumophila Sg6, -10, -12, -13, and -14. Indeed, the wzm and wzt genes were present only in the Sg1 LPS gene cluster, which showed a very specific gene content with respect to the other five serogroups investigated. Based on this observation, we designed primers and developed a classical and a real-time PCR method for the detection and simultaneous identification of L. pneumophila Sg1 in clinical and environmental isolates. Evaluation of the selected primers with 454 Legionella and 38 non-Legionella strains demonstrated 100% specificity. Sensitivity, specificity, and predictive values were further evaluated with 209 DNA extracts from water samples of hospital water supply systems and with 96 respiratory specimens. The results showed that the newly developed quantitative Sg1-specific PCR method is a highly specific and efficient tool for the surveillance and rapid detection of high-risk L. pneumophila Sg1 in water and clinical samples.
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