ArticleJournal of virological methods2011
Detection and differentiation of virulent and avirulent strains of Newcastle disease virus by real-time PCR.
Article in Journal of virological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Optimization and Validation of Universal Real-Time RT-PCR Assay to Detect Virulent Newcastle Disease Viruses.Viruses · 2025Article
- Evaluation of an immunochromatographic strip for detection of avian avulavirus 1 (Newcastle disease virus).Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Detection of Newcastle disease virus minor genetic variants by modified single-stranded conformational polymorphism analysis.BioMed research international · 2014Article
- Newcastle disease virus interaction in targeted therapy against proliferation and invasion pathways of glioblastoma multiforme.BioMed research international · 2014Review
- Detection of avian influenza virus and newcastle disease virus by duplex one step RT PCR.Central European journal of biology · 2013Article
- Avian paramyxovirus serotype-1: a review of disease distribution, clinical symptoms, and laboratory diagnostics.Veterinary medicine international · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid diagnostic method based on the melting curve SYBR Green I real-time PCR analysis was developed to detect and differentiate Newcastle disease virus (NDV) strains. Degenerated primers based on the cleavage site sequence of the F0 gene were designed to detect specific sequences characteristic of virulent and avirulent strains of NDV. Eighteen strains of NDV from four lineages were identified and grouped into virulent and avirulent strains. Peaks on the melting temperature graph with melting temperature values between 80.00 and 83.80°C were observed for lentogenic (avirulent) strains. T(m) values higher than 83.80 were observed for virulent (mesogenic and velogenic) strains. The detection limit of real-time PCR was 2 × 10(2) plasmid copies per reaction or 10(2) EID(50) for velogenic strains and 10(3) EID(50) for lentogenic strains. The results obtained in this study demonstrate the possible applications for melting curve real-time PCR analysis in laboratory practice for the diagnosis and differentiation of avirulent and virulent strains of Newcastle disease virus.
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