ArticleVirology journal2010
Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR.
Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- A descriptive analysis of Streptococcus suis-associated disease in Irish pigs from 2010 to 2024: serotypes, pathology, and antimicrobial resistance.Irish veterinary journal · 2026Article
- CRISPR/Cas12a-assisted visual and on-site detection of porcine circovirus type 2.BMC veterinary research · 2026Article
- Establishment and preliminary application of duplex fluorescence quantitative PCR for porcine circoviruses type 2 and type 3.Heliyon · 2024Article
- Implementation of point-of-care platforms for rapid detection of porcine circovirus type 2.Journal of veterinary science · 2024Article
- Revisiting Porcine Circovirus Infection: Recent Insights and Its Significance in the Piggery Sector.Vaccines · 2023Review
- Virological and Parasitological Characterization of Mini-LEWE Minipigs Using Improved Screening Methods and an Overview of Data on Various Minipig Breeds.Microorganisms · 2021Article
- Development of a multiplex PCR to detect and discriminate porcine circoviruses in clinical specimens.BMC infectious diseases · 2019Article
- Prevalence of porcine circovirus type 3 in pigs in the southeastern Chinese province of Zhejiang.BMC veterinary research · 2019Article
- Co-infection with porcine bocavirus and porcine circovirus 2 affects inflammatory cytokine production and tight junctions of IPEC-J2 cells.Virus genes · 2018Article
- Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2.Virusdisease · 2018Article
- Loop-mediated isothermal amplification for the detection of goose circovirus.Virology journal · 2012Article
- The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus.Virology journal · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine circovirus type 2 (PCV2) and the associated disease postweaning multisystemic wasting syndrome (PMWS) have caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS. To establish a sensitive, specific assay for the detection and quantitation of PCV2, we designed and synthesized specific primers and a probe in the open reading frame 2. The assay had a wide dynamic range with excellent linearity and reliable reproducibility, and detected between 102 and 1010 copies of the genomic DNA per reaction. The coefficient of variation for Ct values varied from 0.59% to 1.05% in the same assay and from 1.9% to 4.2% in 10 different assays. The assay did not cross-react with porcine circovirus type 1, porcine reproductive and respiratory, porcine epidemic diarrhea, transmissible gastroenteritis of pigs and rotavirus. The limits of detection and quantitation were 10 and 100 copies, respectively. Using the established real-time PCR system, 39 of the 40 samples we tested were detected as positive.
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