ArticleAnalytical chemistry2011
Screening natural products for inhibitors of quinone reductase-2 using ultrafiltration LC-MS.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 76 citations in OpenAlex.
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- Progress of isolation, chemical synthesis and biological activities of natural chalcones bearing 2-hydroxy-3-methyl-3-butenyl group.Frontiers in chemistry · 2022Review
- Potential Multifunctional Bioactive Compounds fromJournal of inflammation research · 2022Article
- Drug discovery from natural products using affinity selection-mass spectrometry.Drug discovery today. Technologies · 2021Review
- G protein-coupled receptors: structure- and function-based drug discovery.Signal transduction and targeted therapy · 2021Review
- Recent advances in screening active components from natural products based on bioaffinity techniques.Acta pharmaceutica Sinica. B · 2020Review
- High-throughput identification of G protein-coupled receptor modulators through affinity mass spectrometry screening.Chemical science · 2018Article
- Role of Quinone Reductase 2 in the Antimalarial Properties of Indolone-Type Derivatives.Molecules (Basel, Switzerland) · 2017Article
- Ligand Fishing: A Remarkable Strategy for Discovering Bioactive Compounds from Complex Mixture of Natural Products.Molecules (Basel, Switzerland) · 2016Review
- Comparison of analytical techniques for the identification of bioactive compounds from natural products.Natural product reports · 2016Review
- Isolation of α-Amylase Inhibitors from Kadsura longipedunculata Using a High-Speed Counter-Current Chromatography Target Guided by Centrifugal Ultrafiltration with LC-MS.Molecules (Basel, Switzerland) · 2016Article
- Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches.Scientific reports · 2016Article
- Identification of Eupatilin from Artemisia argyi as a Selective PPARα Agonist Using Affinity Selection Ultrafiltration LC-MS.Molecules (Basel, Switzerland) · 2015Article
- A ligand-observed mass spectrometry approach integrated into the fragment based lead discovery pipeline.Scientific reports · 2015Article
- Matching the power of high throughput screening to the chemical diversity of natural products.Natural product reports · 2013Review
- Review
- Identification of Streptomyces sp. nov. WH26 producing cytotoxic compounds isolated from marine solar saltern in China.World journal of microbiology & biotechnology · 2013Article
- Resveratrol derivatives as promising chemopreventive agents with improved potency and selectivity.Molecular nutrition & food research · 2011Article
Corrections and comments
- Erratum issuedvan Breemen, Richard R [corrected to van Breemen, Richard B]
Authors and funding
16 authors at 6 institutions in 1 country.
Funding
Abstract
Inhibitors of quinone reductase-2 (NQO2; QR-2) can have antimalarial activity and antitumor activities or can function as chemoprevention agents by preventing the metabolic activation of toxic quinones such as menadione. To expedite the search for new natural product inhibitors of QR-2, we developed a screening assay based on ultrafiltration liquid chromatography-mass spectrometry that is compatible with complex samples such as bacterial or botanical extracts. Human QR-2 was prepared recombinantly, and the known QR-2 inhibitor, resveratrol, was used as a positive control and as a competitive ligand to eliminate false positives. Ultrafiltration LC-MS screening of extracts of marine sediment bacteria resulted in the discovery of tetrangulol methyl ether as an inhibitor of QR-2. When applied to the screening of hop extracts from the botanical, Humulus lupulus L., xanthohumol and xanthohumol D were identified as ligands of QR-2. Inhibition of QR-2 by these ligands was confirmed using a functional enzyme assay. Furthermore, binding of xanthohumol and xanthohumol D to the active site of QR-2 was confirmed using X-ray crystallography. Ultrafiltration LC-MS was shown to be a useful assay for the discovery of inhibitors of QR-2 in complex matrixes such as extracts of bacteria and botanicals.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.