ArticleAnalytical chemistry2011
Capillary isoelectric focusing of individual mitochondria.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Micro- and Nano-Devices for Studying Subcellular Biology.Small (Weinheim an der Bergstrasse, Germany) · 2021Review
- Capillary Electrophoresis with Laser-Induced Fluorescent Detection of Immunolabeled Individual Autophagy Organelles Isolated from Liver Tissue.Analytical chemistry · 2016Article
- Nanohole Array-Directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis.Analytical chemistry · 2015Article
- Simultaneous measurement of individual mitochondrial membrane potential and electrophoretic mobility by capillary electrophoresis.Analytical chemistry · 2014Article
- Analysis of individual mitochondria via fluorescent immunolabeling with Anti-TOM22 antibodies.Analytical and bioanalytical chemistry · 2014Article
- Describing autophagy via analysis of individual organelles by capillary electrophoresis with laser induced fluorescence detection.Analytical chemistry · 2013Article
- High sensitivity capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for the rapid analysis of complex proteomes.Current opinion in chemical biology · 2013Review
- Predicting isoelectric points of nonfunctional mitochondria from Monte Carlo simulations of surface compositions.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Review on recent advances in the analysis of isolated organelles.Analytica chimica acta · 2012Review
- Analytical tools for characterizing heterogeneity in organelle content.Current opinion in chemical biology · 2012Review
- Individual organelle pH determinations of magnetically enriched endocytic organelles via laser-induced fluorescence detection.Analytical chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Mitochondria are highly heterogeneous organelles that likely have unique isoelectric points (pI), which are related to their surface compositions and could be exploited in their purification and isolation. Previous methods to determine pI of mitochondria report an average pI. This article is the first report of the determination of the isoelectric points of individual mitochondria by capillary isoelectric focusing (cIEF). In this method, mitochondria labeled with the mitochondrial-specific probe 10-N-nonyl acridine orange (NAO) are injected into a fused-silica capillary in a solution of carrier ampholytes at physiological pH and osmolarity, where they are focused then chemically mobilized and detected by laser-induced fluorescence (LIF). Fluorescein-derived pI markers are used as internal standards to assign a pI value to each individually detected mitochondrial event, and a mitochondrial pI distribution is determined. This method provides reproducible distributions of individual mitochondrial pI, accurate determination of the pI of individual mitochondria by the use of internal standards, and resolution of 0.03 pH units between individual mitochondria. This method could also be applied to investigate or design separations of organelle subtypes (e.g., subsarcolemmal and interfibrillar skeletal muscle mitochondria) and to determine the pIs of other biological or nonbiological particles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.