ArticleJournal of lipid research2011
Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.
Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Preparation of Plasma Membrane Vesicles, Lysosomes, Proteoliposomes and Alternative Sample Types for SSM-Based Electrophysiology.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The selenoprotein P/ApoER2 axis facilitates selenium accumulation in selenoprotein P-accepting cells and confers prolonged resistance to ferroptosis.Redox biology · 2025Article
- Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses.EBioMedicine · 2019Article
- The CCT chaperonin is a novel regulator of CaScience advances · 2018Article
- Role of Membrane Cholesterol Levels in Activation of Lyn upon Cell Detachment.International journal of molecular sciences · 2018Review
- Carboxypeptidase O is a lipid droplet-associated enzyme able to cleave both acidic and polar C-terminal amino acids.PloS one · 2018Article
- PtdIns4KIIα generates endosomal PtdIns(4)P and is required for receptor sorting at early endosomes.Molecular biology of the cell · 2016Article
- Subcellular fractionation method to study endosomal trafficking of Kaposi's sarcoma-associated herpesvirus.Cell & bioscience · 2016Article
- Chromosomal Instability and Phosphoinositide Pathway Gene Signatures in Glioblastoma Multiforme.Molecular neurobiology · 2016Article
- Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection.Molecular & cellular proteomics : MCP · 2015Article
- Adaptive lipid packing and bioactivity in membrane domains.PloS one · 2015Article
- Article
- The serine/threonine kinase Ndr2 controls integrin trafficking and integrin-dependent neurite growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Raft-like membranes from the trans-Golgi network and endosomal compartments.Nature protocols · 2013Article
- CLN3 loss disturbs membrane microdomain properties and protein transport in brain endothelial cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Mammalian phosphatidylinositol 4-kinases as modulators of membrane trafficking and lipid signaling networks.Progress in lipid research · 2013Review
- ATP11B mediates platinum resistance in ovarian cancer.The Journal of clinical investigation · 2013Article
- Phosphatidylinositol 4-kinases and PI4P metabolism in the nervous system: roles in psychiatric and neurological diseases.Molecular neurobiology · 2013Review
- The asymmetrical structure of Golgi apparatus membranes revealed by in situ atomic force microscope.PloS one · 2013Article
- Phosphatidylinositol 4-kinase IIα is palmitoylated by Golgi-localized palmitoyltransferases in cholesterol-dependent manner.The Journal of biological chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Cholesterol is an abundant lipid of the trans-Golgi network (TGN) and of certain endosomal membranes where cholesterol-rich microdomains are important in the organization and compartmentalization of vesicular trafficking. Here we describe the development of a rapid method to isolate a cholesterol-rich endomembrane fraction. We show that widely used subcellular fractionation techniques incompletely separate cholesterol-rich membranes, such as the TGN, from organelles, such as late endosomes and lysosomes. To address this issue, we devised a new subcellular fractionation scheme involving two rounds of velocity centrifugation, membrane sonication, and discontinuous sucrose density gradient centrifugation. This strategy resulted in the isolation of a cholesterol and GM1 glycosphingolipid-enriched membrane fraction that was completely cleared of plasma membrane, endoplasmic reticulum, and mitochondria. This buoyant fraction was enriched for the TGN and recycling endosome proteins Rab11 and syntaxin-6, and it was well resolved from cis-Golgi and early and late endosomal membranes. We demonstrate that this technique can give useful insights into the compartmentation of phosphoinositide synthesis, and it facilitates the isolation of cholesterol-rich membranes from a population of TGN-trafficking vesicles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.