ArticleJournal of virology2011
Rhesus rotavirus entry into a polarized epithelium is endocytosis dependent and involves sequential VP4 conformational changes.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Rotavirus Spike Protein VP4 Mediates Viroplasm Assembly by Association to Actin Filaments.Journal of virology · 2022Article
- Treading a HOSTile path: Mapping the dynamic landscape of host cell-rotavirus interactions to explore novel host-directed curative dimensions.Virulence · 2021Review
- Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II.Veterinary research · 2018Article
- Histo-blood group antigen-binding specificities of human rotaviruses are associated with gastroenteritis but not with in vitro infection.Scientific reports · 2018Article
- Most rotavirus strains require the cation-independent mannose-6-phosphate receptor, sortilin-1, and cathepsins to enter cells.Virus research · 2018Article
- Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.PLoS pathogens · 2018Article
- Drebrin restricts rotavirus entry by inhibiting dynamin-mediated endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Rotavirus entry: a deep journey into the cell with several exits.Journal of virology · 2015Review
- Structural correlates of rotavirus cell entry.PLoS pathogens · 2014Article
- Carbohydrate recognition by rotaviruses.Journal of structural and functional genomics · 2014Review
- New insights into rotavirus entry machinery: stabilization of rotavirus spike conformation is independent of trypsin cleavage.PLoS pathogens · 2014Article
- Relative roles of GM1 ganglioside, N-acylneuraminic acids, and α2β1 integrin in mediating rotavirus infection.Journal of virology · 2014Article
- Genome-wide RNAi screen reveals a role for the ESCRT complex in rotavirus cell entry.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Interferon-inducible transmembrane protein 3 (IFITM3) restricts reovirus cell entry.The Journal of biological chemistry · 2013Article
- Mutations in the rotavirus spike protein VP4 reduce trypsin sensitivity but not viral spread.The Journal of general virology · 2013Article
- The spike protein VP4 defines the endocytic pathway used by rotavirus to enter MA104 cells.Journal of virology · 2013Article
- Interactions among capsid proteins orchestrate rotavirus particle functions.Current opinion in virology · 2012Review
- Rhesus rotavirus trafficking during entry into MA104 cells is restricted to the early endosome compartment.Journal of virology · 2012Article
- Structural insights into the coupling of virion assembly and rotavirus replication.Nature reviews. Microbiology · 2012Review
- Cross-linking of rotavirus outer capsid protein VP7 by antibodies or disulfides inhibits viral entry.Journal of virology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Rotavirus (RV) cell entry is an incompletely understood process, involving VP4 and VP7, the viral proteins composing the outermost layer of the nonenveloped RV triple-layered icosahedral particle (TLP), encasing VP6. VP4 can exist in three conformational states: soluble, cleaved spike, and folded back. In order to better understand the events leading to RV entry, we established a detection system to image input virus by monitoring the rhesus RV (RRV) antigens VP4, VP6, and VP7 at very early times postinfection. We provide evidence that decapsidation occurs directly after cell membrane penetration. We also demonstrate that several VP4 and VP7 conformational changes take place during entry. In particular, we detected, for the first time, the generation of folded-back VP5 in the context of the initiation of infection. Folded-back VP5 appears to be limited to the entry step. We furthermore demonstrate that RRV enters the cell cytoplasm through an endocytosis pathway. The endocytosis hypothesis is supported by the colocalization of RRV antigens with the early endosome markers Rab4 and Rab5. Finally, we provide evidence that the entry process is likely dependent on the endocytic Ca(2+) concentration, as bafilomycin A1 treatment as well as an augmentation of the extracellular calcium reservoir using CaEGTA, which both lead to an elevated intraendosomal calcium concentration, resulted in the accumulation of intact virions in the actin network. Together, these findings suggest that internalization, decapsidation, and cell membrane penetration involve endocytosis, calcium-dependent uncoating, and VP4 conformational changes, including a fold-back.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.