ArticleBiophysical journal2011
Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 69 citations in OpenAlex.
- Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue.Analytical chemistry · 2024Article
- Toward Gene-Correlated Spatially Resolved Metabolomics with Fingerprint Coherent Raman Imaging.The journal of physical chemistry. B · 2023Article
- Evaluation of Chemical Changes in Laboratory-Induced Colistin-ResistantInternational journal of molecular sciences · 2021Article
- Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation.Molecules (Basel, Switzerland) · 2020Review
- Parallelized shifted-excitation Raman difference spectroscopy for fluorescence rejection in a temporary varying system.Journal of biophotonics · 2019Article
- Immune cell type, cell activation, and single cell heterogeneity revealed by label-free optical methods.Scientific reports · 2019Article
- Graphene Microelectrode Arrays for Electrical and Optical Measurements of Human Stem Cell-Derived Cardiomyocytes.Cellular and molecular bioengineering · 2018Article
- Nanobiosensing Platforms for Real-Time and Non-Invasive Monitoring of Stem Cell Pluripotency and Differentiation.Sensors (Basel, Switzerland) · 2018Review
- Label-Free Raman Hyperspectral Imaging of Single Cells Cultured on Polymer Substrates.Applied spectroscopy · 2017Article
- Steps toward Maturation of Embryonic Stem Cell-Derived Cardiomyocytes by Defined Physical Signals.Stem cell reports · 2017Article
- Label-free Raman spectroscopic imaging to extract morphological and chemical information from a formalin-fixed, paraffin-embedded rat colon tissue section.International journal of experimental pathology · 2016Article
- Non-invasive Chamber-Specific Identification of Cardiomyocytes in Differentiating Pluripotent Stem Cells.Stem cell reports · 2016Article
- Detection of early stage changes associated with adipogenesis using Raman spectroscopy under aseptic conditions.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.Scientific reports · 2015Article
- Raman spectral dynamics of single cells in the early stages of growth factor stimulation.Biophysical journal · 2015Article
- Raman and autofluorescence spectrum dynamics along the HRG-induced differentiation pathway of MCF-7 cells.Biophysical journal · 2014Article
- Visualizing cell state transition using Raman spectroscopy.PloS one · 2014Article
- Raman spectroscopy in biomedicine - non-invasive in vitro analysis of cells and extracellular matrix components in tissues.Biotechnology journal · 2013Review
- Label-free separation of human embryonic stem cells and their differentiating progenies by phasor fluorescence lifetime microscopy.Journal of biomedical optics · 2012Article
- Phasor fluorescence lifetime microscopy of free and protein-bound NADH reveals neural stem cell differentiation potential.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Raman microspectroscopy (RMS) was used to detect and image molecular markers specific to cardiomyocytes (CMs) derived from human embryonic stem cells (hESCs). This technique is noninvasive and thus can be used to discriminate individual live CMs within highly heterogeneous cell populations. Principal component analysis (PCA) of the Raman spectra was used to build a classification model for identification of individual CMs. Retrospective immunostaining imaging was used as the gold standard for phenotypic identification of each cell. We were able to discriminate CMs from other phenotypes with >97% specificity and >96% sensitivity, as calculated with the use of cross-validation algorithms (target 100% specificity). A comparison between Raman spectral images corresponding to selected Raman bands identified by the PCA model and immunostaining of the same cells allowed assignment of the Raman spectral markers. We conclude that glycogen is responsible for the discrimination of CMs, whereas myofibril proteins have a lesser contribution. This study demonstrates the potential of RMS for allowing the noninvasive phenotypic identification of hESC progeny. With further development, such label-free optical techniques may enable the separation of high-purity cell populations with mature phenotypes, and provide repeated measurements to monitor time-dependent molecular changes in live hESCs during differentiation in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.