ArticleBiophysical journal2011
Detection of membrane protein two-dimensional crystals in living cells.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- A streamlined approach to structure elucidation using in cellulo crystallized recombinant proteins, InCellCryst.Nature communications · 2024Article
- Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals.Acta crystallographica. Section D, Structural biology · 2016Article
- Article
- Towards protein-crystal centering using second-harmonic generation (SHG) microscopy.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Modeling the SHG activities of diverse protein crystals.Acta crystallographica. Section D, Biological crystallography · 2012Article
- Probing the early temporal and spatial interaction of the Sindbis virus capsid and E2 proteins with reverse genetics.Journal of virology · 2012Article
- Cell-to-cell diversity in a synchronized Chlamydomonas culture as revealed by single-cell analyses.Biophysical journal · 2012Article
- Membrane protein structure determination by electron crystallography.Current opinion in structural biology · 2012Review
- Two-photon excited UV fluorescence for protein crystal detection.Acta crystallographica. Section D, Biological crystallography · 2011Article
- Femtosecond nanocrystallography using X-ray lasers for membrane protein structure determination.Current opinion in structural biology · 2011Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
It is notoriously difficult to grow membrane protein crystals and solve membrane protein structures. Improved detection and screening of membrane protein crystals are needed. We have shown here that second-order nonlinear optical imaging of chiral crystals based on second harmonic generation can provide sensitive and selective detection of two-dimensional protein crystalline arrays with sufficiently low background to enable crystal detection within the membranes of live cells. The method was validated using bacteriorhodopsin crystals generated in live Halobacterium halobium bacteria and confirmed by electron microscopy from the isolated crystals. Additional studies of alphavirus glycoproteins indicated the presence of localized crystalline domains associated with virus budding from mammalian cells. These results suggest that in vivo crystallization may provide a means for expediting membrane protein structure determination for proteins exhibiting propensities for two-dimensional crystal formation.
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