ArticleBiophysical journal2011
X-ray diffraction from membrane protein nanocrystals.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- In Situ XRPD Investigation of Relative Humidity-Induced Lattice Responses in Tetragonal Hen Egg-White Lysozyme.Biomolecules · 2026Article
- Investigations on genomic, topological and structural properties of diguanylate cyclases involved inCurrent research in structural biology · 2025Article
- Blue and red in the protein world: Photoactive yellow protein and phytochromes as revealed by time-resolved crystallography.Structural dynamics (Melville, N.Y.) · 2024Article
- Effects of pre-drying treatment and particle sizes on physicochemical and structural properties of pumpkin flour.Heliyon · 2023Article
- Fixed-target serial crystallography at the Structural Biology Center.Journal of synchrotron radiation · 2022Article
- Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review.Molecules (Basel, Switzerland) · 2019Review
- Time-Resolved Macromolecular Crystallography at Pulsed X-ray Sources.International journal of molecular sciences · 2019Review
- Watching Proteins Function with Time-resolved X-ray Crystallography.Journal of physics D: Applied physics · 2017Article
- Serial femtosecond crystallography opens new avenues for Structural Biology.Protein and peptide letters · 2016Article
- Microfluidic sorting of protein nanocrystals by size for X-ray free-electron laser diffraction.Structural dynamics (Melville, N.Y.) · 2015Article
- Goniometer-based femtosecond X-ray diffraction of mutant 30S ribosomal subunit crystals.Structural dynamics (Melville, N.Y.) · 2015Article
- Precise Manipulation and Patterning of Protein Crystals for Macromolecular Crystallography Using Surface Acoustic Waves.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- High throughput protein nanocrystal fractionation in a microfluidic sorter.Analytical chemistry · 2015Article
- Fixed-target protein serial microcrystallography with an x-ray free electron laser.Scientific reports · 2014Article
- Use of transmission electron microscopy to identify nanocrystals of challenging protein targets.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Crystallization of the large membrane protein complex photosystem I in a microfluidic channel.ACS nano · 2013Article
- Dielectrophoretic sorting of membrane protein nanocrystals.ACS nano · 2013Article
- Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature.Science (New York, N.Y.) · 2013Article
- Room temperature femtosecond X-ray diffraction of photosystem II microcrystals.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Toward structure determination using membrane-protein nanocrystals and microcrystals.Methods (San Diego, Calif.) · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
Membrane proteins constitute > 30% of the proteins in an average cell, and yet the number of currently known structures of unique membrane proteins is < 300. To develop new concepts for membrane protein structure determination, we have explored the serial nanocrystallography method, in which fully hydrated protein nanocrystals are delivered to an x-ray beam within a liquid jet at room temperature. As a model system, we have collected x-ray powder diffraction data from the integral membrane protein Photosystem I, which consists of 36 subunits and 381 cofactors. Data were collected from crystals ranging in size from 100 nm to 2 μm. The results demonstrate that there are membrane protein crystals that contain < 100 unit cells (200 total molecules) and that 3D crystals of membrane proteins, which contain < 200 molecules, may be suitable for structural investigation. Serial nanocrystallography overcomes the problem of x-ray damage, which is currently one of the major limitations for x-ray structure determination of small crystals. By combining serial nanocrystallography with x-ray free-electron laser sources in the future, it may be possible to produce molecular-resolution electron-density maps using membrane protein crystals that contain only a few hundred or thousand unit cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.