ArticlePlant molecular biology2011
Recognition of A. thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein.
Article in Plant molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Decoding allelic diversity, transcript variants and transcriptional complexity of CENH3 gene in Brassica oleracea var. botrytis.Protoplasma · 2023Article
- The Centromere Histone Is Conserved and Associated with Tandem Repeats Sharing a Conserved 19-bp Box in the Holocentromere of Meloidogyne Nematodes.Molecular biology and evolution · 2021Article
- Unequal contribution of two paralogous CENH3 variants in cowpea centromere function.Communications biology · 2020Article
- High allelic diversity of the centromere-specific histone H3 (CENH3) in the legume sainfoin (Onobrychis viciifolia).Molecular biology reports · 2020Article
- Centromere histone H3- and phospholipase-mediated haploid induction in plants.Plant methods · 2019Review
- Recurrent Gene Duplication Leads to Diverse Repertoires of Centromeric Histones in Drosophila Species.Molecular biology and evolution · 2017Article
- Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive.Annals of botany · 2016Article
- The distribution of α-kleisin during meiosis in the holocentromeric plant Luzula elegans.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
- Epigenetic Histone Marks of Extended Meta-Polycentric Centromeres of Lathyrus and Pisum Chromosomes.Frontiers in plant science · 2016Article
- Cenh3: An Emerging Player in Haploid Induction Technology.Frontiers in plant science · 2016Review
- Centromeres Off the Hook: Massive Changes in Centromere Size and Structure Following Duplication of CenH3 Gene in Fabeae Species.Molecular biology and evolution · 2015Article
- The differential loading of two barley CENH3 variants into distinct centromeric substructures is cell type- and development-specific.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- Atypical centromeres in plants-what they can tell us.Frontiers in plant science · 2015Review
- Naturally occurring differences in CENH3 affect chromosome segregation in zygotic mitosis of hybrids.PLoS genetics · 2015Article
- Centromeres and kinetochores of Brassicaceae.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Review
- Chromosome segregation in plant meiosis.Frontiers in plant science · 2014Review
- Chromosome dynamics visualized with an anti-centromeric histone H3 antibody in Allium.PloS one · 2012Article
- Stretching the rules: monocentric chromosomes with multiple centromere domains.PLoS genetics · 2012Article
- Color recovery after photoconversion of H2B::mEosFP allows detection of increased nuclear DNA content in developing plant cells.Plant physiology · 2012Article
- Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The centromere is an essential chromosomal component assembling the kinetochore for chromosome attachment to the spindle microtubules and for directing the chromosome segregation during nuclear division. Kinetochore assembly requires deposition of the centromeric histone H3 variant (CENH3) into centromeric nucleosomes. CENH3 has a variable N-terminal and a more conserved C-terminal part, including the loop1 region of the histone fold domain, which is considered to be critical for centromere targeting. To investigate the structural requirements for centromere targeting, constructs for EYFP-tagged CENH3 of A. lyrata, A. arenosa, Capsella bursa-pastoris, Zea mays and Luzula nivea (the latter with holocentric chromosomes) were transformed into A. thaliana. Except for LnCENH3, all recombinant CENH3 proteins targeted A. thaliana centromeres, but the more distantly related the heterologous protein is, the lower is the efficiency of targeting. Alignment of CENH3 sequences revealed that the tested species share only three amino acids at loop1 region: threonine2, arginine12 and alanine15. These three amino acids were substituted by asparagine, proline and valine encoding sequences within a recombinant EYFP-AtCENH3 construct via PCR mutagenesis prior to transformation of A. thaliana. After transformation, immunostaining of root tip nuclei with anti-GFP antibodies yielded only diffuse signals, indicating that the original three amino acids are necessary but not sufficient for targeting A. thaliana centromeres.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.