ReviewCellular and molecular life sciences : CMLS2011
Mining electron density for functionally relevant protein polysterism in crystal structures.
Review in Cellular and molecular life sciences : CMLS, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Ubiquitin's Conformational Heterogeneity as Discerned by Nuclear Magnetic Resonance Spectroscopy.Chembiochem : a European journal of chemical biology · 2024Review
- Changes in Active Site Loop Conformation Relate to the Transition toward a Novel Enzymatic Activity.JACS Au · 2024Article
- Synergism between x-ray crystallography and NMR residual dipolar couplings in characterizing protein dynamics.Structural dynamics (Melville, N.Y.) · 2023Article
- Ensemble-function relationships to dissect mechanisms of enzyme catalysis.Science advances · 2022Article
- Instrumentation and experimental procedures for robust collection of X-ray diffraction data from protein crystals across physiological temperatures.Journal of applied crystallography · 2020Article
- A mechanistic view of enzyme evolution.Protein science : a publication of the Protein Society · 2020Review
- Conformational sampling and kinetics changes across a non-Arrhenius break point in the enzyme thermolysin.Structural dynamics (Melville, N.Y.) · 2020Article
- A chemical interpretation of protein electron density maps in the worldwide protein data bank.PloS one · 2020Article
- The role of protein dynamics in the evolution of new enzyme function.Nature chemical biology · 2016Article
- Molecular dynamics simulation of triclinic lysozyme in a crystal lattice.Protein science : a publication of the Protein Society · 2016Article
- Predicting X-ray diffuse scattering from translation-libration-screw structural ensembles.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Exploring the role of conformational heterogeneity in cis-autoproteolytic activation of ThnT.Biochemistry · 2014Article
- Automated identification of functional dynamic contact networks from X-ray crystallography.Nature methods · 2013Article
- Peptide crystal simulations reveal hidden dynamics.Journal of the American Chemical Society · 2013Article
- Accessing protein conformational ensembles using room-temperature X-ray crystallography.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
2 authors.
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Abstract
This review focuses on conceptual and methodological advances in our understanding and characterization of the conformational heterogeneity of proteins. Focusing on X-ray crystallography, we describe how polysterism, the interconversion of pre-existing conformational substates, has traditionally been analyzed by comparing independent crystal structures or multiple chains within a single crystal asymmetric unit. In contrast, recent studies have focused on mining electron density maps to reveal previously 'hidden' minor conformational substates. Functional tests of the importance of minor states suggest that evolutionary selection shapes the entire conformational landscape, including uniquely configured conformational substates, the relative distribution of these substates, and the speed at which the protein can interconvert between them. An increased focus on polysterism may shape the way protein structure and function is studied in the coming years.
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