ArticleCurrent microbiology2011
Identification of Pythium insidiosum by nested PCR in cutaneous lesions of Brazilian horses and rabbits.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
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- Leptospira borgptersenii and Leptospira interrogans identified in wild mammals in Rio Grande do Sul, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
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- Leptospira interrogans in bats in Rio Grande do Sul State, Brazil: epidemiologic aspects and phylogeny.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Nested PCR Detection ofVeterinary sciences · 2022Article
- Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis.Journal of fungi (Basel, Switzerland) · 2021Article
- Efficacy of Azithromycin and Miltefosine in Experimental Systemic Pythiosis in Immunosuppressed Mice.Antimicrobial agents and chemotherapy · 2019Article
- Article
- Biochemical and genetic analyses of the oomycetePeerJ · 2018Article
- Chemically induced disseminated pythiosis in BALB/c mice: A new experimental model for Pythium insidiosum infection.PloS one · 2017Article
- Diagnosis and Treatment of Pythium Insidiosum Corneal Ulcer in a Chinese Child: A Case Report and Literature Review.The American journal of case reports · 2016Review
- Cutaneous Pythiosis in calves: An epidemiologic, pathologic, serologic and molecular characterization.Medical mycology case reports · 2016Article
- In Vitro Synergism between Azithromycin or Terbinafine and Topical Antimicrobial Agents against Pythium insidiosum.Antimicrobial agents and chemotherapy · 2016Article
- In Vitro and In Vivo Antimicrobial Activities of Minocycline in Combination with Azithromycin, Clarithromycin, or Tigecycline against Pythium insidiosum.Antimicrobial agents and chemotherapy · 2016Article
- Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis.Journal of clinical microbiology · 2016Article
- Combat-Related Pythium aphanidermatum Invasive Wound Infection: Case Report and Discussion of Utility of Molecular Diagnostics.Journal of clinical microbiology · 2015Article
- New insights into the in vitro susceptibility of Pythium insidiosum.Antimicrobial agents and chemotherapy · 2014Article
- In vitro synergism observed with azithromycin, clarithromycin, minocycline, or tigecycline in association with antifungal agents against Pythium insidiosum.Antimicrobial agents and chemotherapy · 2014Article
- In vitro reproduction of the life cycle of Pythium insidiosum from kunkers' equine and their role in the epidemiology of pythiosis.Mycopathologia · 2014Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pythium insidiosum is a fungus-like organism present in subtropical and tropical areas, such as Brazil, known to infect humans and various animal species. P. insidiosum is the etiological agent of pythiosis, an emerging and granulomatous disease characterized mainly by cutaneous and subcutaneous lesions in horses, the principal species affected. Accurate diagnosis of pythiosis and identification of its causal agent by microbiological and serological tests can be often difficult and inconclusive principally for horses and humans. The aim of this study was to evaluate the application of the previously described P. insidiosum-specific nested polymerase chain reaction (PCR) assay to directly detect P. insidiosum DNA in clinical and experimental lesions. Universal fungal primers (ITS1 and ITS4) were used during the first-round of PCR to amplify ITS1, 5.8s, and ITS2. A second-round of PCR was conducted with P. insidiosum-specific primers (PI1 and PI2) to amplify a variable region within this ITS1. In this study, a total of 21 equine clinical samples (kunkers) and 28 specimens from experimentally infected rabbits were analyzed by nested PCR. The first-round of PCR generated 800-base pair products, and the second-round produced 105-base pair amplicons for each P. insidiosum-specific sample; no amplicons were generated in negative control samples. Our results suggest that nested PCR is an important and efficient tool for diagnosis of both endemic (horse samples) and experimental (rabbit samples) pythiosis.
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