ArticlePLoS genetics2010
A single enhancer regulating the differential expression of duplicated red-sensitive opsin genes in zebrafish.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 79 citations in OpenAlex.
- A potential cis-regulatory element regulates differential expression of long wavelength-sensitive opsins in zebrafish.Scientific reports · 2026Article
- Article
- Differential Localization and Functional Roles of mGluR6 Paralogs in Zebrafish Retina.Investigative ophthalmology & visual science · 2024Article
- Glutamate transporters are involved in direct inhibitory synaptic transmission in the vertebrate retina.Open biology · 2024Article
- Retinoic acid signaling regulates spatiotemporal specification of human green and red cones.PLoS biology · 2024Article
- Plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure.Scientific reports · 2023Article
- Rho enhancers play unexpectedly minor roles in Rhodopsin transcription and rod cell integrity.Scientific reports · 2023Article
- Long wavelength-sensing cones of zebrafish retina exhibit multiple layers of transcriptional heterogeneity.Frontiers in cellular neuroscience · 2023Article
- The evolutionary history and spectral tuning of vertebrate visual opsins.Developmental biology · 2023Review
- Correlated Expression of the Opsin Retrogene LWS-R and its Host Gene in Two Poeciliid Fishes.Zoological studies · 2022Article
- Evidence of regional specializations in regenerated zebrafish retina.Experimental eye research · 2021Review
- Foxq2 determines blue cone identity in zebrafish.Science advances · 2021Article
- Ganglion cells in larval zebrafish retina integrate inputs from multiple cone types.Journal of neurophysiology · 2021Article
- Axes of visual adaptation in the ecologically diverse family Cichlidae.Seminars in cell & developmental biology · 2020Review
- Tbx2a Modulates Switching of RH2 and LWS Opsin Gene Expression.Molecular biology and evolution · 2020Article
- Thyroid hormone receptors mediate two distinct mechanisms of long-wavelength vision.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish).PLoS genetics · 2020Article
- Identification and Characterization of Cis-Regulatory Elements for Photoreceptor-Type-Specific Transcription in ZebraFish.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Endocrine regulation of multichromatic color vision.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Strain variations in cone wavelength peaksVisual neuroscience · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fundamental step in the evolution of the visual system is the gene duplication of visual opsins and differentiation between the duplicates in absorption spectra and expression pattern in the retina. However, our understanding of the mechanism of expression differentiation is far behind that of spectral tuning of opsins. Zebrafish (Danio rerio) have two red-sensitive cone opsin genes, LWS-1 and LWS-2. These genes are arrayed in a tail-to-head manner, in this order, and are both expressed in the long member of double cones (LDCs) in the retina. Expression of the longer-wave sensitive LWS-1 occurs later in development and is thus confined to the peripheral, especially ventral-nasal region of the adult retina, whereas expression of LWS-2 occurs earlier and is confined to the central region of the adult retina, shifted slightly to the dorsal-temporal region. In this study, we employed a transgenic reporter assay using fluorescent proteins and P1-artificial chromosome (PAC) clones encompassing the two genes and identified a 0.6-kb "LWS-activating region" (LAR) upstream of LWS-1, which regulates expression of both genes. Under the 2.6-kb flanking upstream region containing the LAR, the expression pattern of LWS-1 was recapitulated by the fluorescent reporter. On the other hand, when LAR was directly conjugated to the LWS-2 upstream region, the reporter was expressed in the LDCs but also across the entire outer nuclear layer. Deletion of LAR from the PAC clones drastically lowered the reporter expression of the two genes. These results suggest that LAR regulates both LWS-1 and LWS-2 by enhancing their expression and that interaction of LAR with the promoters is competitive between the two genes in a developmentally restricted manner. Sharing a regulatory region between duplicated genes could be a general way to facilitate the expression differentiation in duplicated visual opsins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.