ArticleProceedings of the National Academy of Sciences of the United States of America2011
Vibrio cholerae anaerobic induction of virulence gene expression is controlled by thiol-based switches of virulence regulator AphB.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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77 citing papers in PubMed, 113 citations in OpenAlex.
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- Host-derived O-glycans inhibit toxigenic conversion by a virulence-encoding phage in Vibrio cholerae.The EMBO journal · 2023Article
- Structural Insights into Regulation of Vibrio Virulence Gene Networks.Advances in experimental medicine and biology · 2023Article
- PAS Domain-Containing Chemoreceptors Influence the Signal Sensing and Intestinal Colonization ofGenes · 2022Article
- S-Nitrosylation of the virulence regulator AphB promotes Vibrio cholerae pathogenesis.PLoS pathogens · 2022Article
- ToxT Regulon Is Nonessential for Vibrio cholerae Colonization in Adult Mice.Applied and environmental microbiology · 2022Article
- Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens.Cell reports · 2021Article
- Bile Salts Promote ToxR Regulon Activation during Growth under Virulence-Inducing Conditions.Infection and immunity · 2021Article
- Vibrio cholerae TolC Is Required for Expression of the ToxR Regulon.Infection and immunity · 2021Article
- CBS-derived H2S facilitates host colonization of Vibrio cholerae by promoting the iron-dependent catalase activity of KatB.PLoS pathogens · 2021Article
- ToxR Mediates the Antivirulence Activity of Phenyl-Arginine-β-Naphthylamide To Attenuate Vibrio cholerae Virulence.Infection and immunity · 2021Article
- Positive regulation of Type III secretion effectors and virulence by RyhB paralogs in Salmonella enterica serovar Enteritidis.Veterinary research · 2021Article
- On or Off: Life-Changing Decisions Made byInfectious microbes & diseases · 2020Review
- Pathogenicity and virulence regulation ofVirulence · 2020Review
17 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Bacterial pathogens have evolved sophisticated signal transduction systems to coordinately control the expression of virulence determinants. For example, the human pathogen Vibrio cholerae is able to respond to host environmental signals by activating transcriptional regulatory cascades. The host signals that stimulate V. cholerae virulence gene expression, however, are still poorly understood. Previous proteomic studies indicated that the ambient oxygen concentration plays a role in V. cholerae virulence gene expression. In this study, we found that under oxygen-limiting conditions, an environment similar to the intestines, V. cholerae virulence genes are highly expressed. We show that anaerobiosis enhances dimerization and activity of AphB, a transcriptional activator that is required for the expression of the key virulence regulator TcpP, which leads to the activation of virulence factor production. We further show that one of the three cysteine residues in AphB, C(235), is critical for oxygen responsiveness, as the AphB(C235S) mutant can activate virulence genes under aerobic conditions in vivo and can bind to tcpP promoters in the absence of reducing agents in vitro. Mass spectrometry analysis suggests that under aerobic conditions, AphB is modified at the C(235) residue. This modification is reversible between oxygen-rich aquatic environments and oxygen-limited human hosts, suggesting that V. cholerae may use a thiol-based switch mechanism to sense intestinal signals and activate virulence.
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