ArticleJournal of molecular biology2011
Construction of a genetic multiplexer to toggle between chemosensory pathways in Escherichia coli.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Modular Scalable Synthetic Gene Circuits for Complex Functions Within Minimal Computational Layers in Human Cells.Nature communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Gene circuit-based sensors.Fundamental research · 2025Review
- Achieving spatially precise diagnosis and therapy in the mammalian gut using synthetic microbial gene circuits.Frontiers in bioengineering and biotechnology · 2022Review
- Modelling co-translational dimerization for programmable nonlinearity in synthetic biology.Journal of the Royal Society, Interface · 2020Article
- Characterization and synthetic biology of lipase from Bacillus amyloliquefaciens strain.Archives of microbiology · 2020Article
- High-Performance Biocomputing in Synthetic Biology-Integrated Transcriptional and Metabolic Circuits.Frontiers in bioengineering and biotechnology · 2019Article
- Dynamic control of endogenous metabolism with combinatorial logic circuits.Molecular systems biology · 2018Article
- Spatial interference scale as a determinant of microbial range expansion.Science advances · 2018Article
- Engineering microbial consortia for controllable outputs.The ISME journal · 2016Article
- Molecular recordings by directed CRISPR spacer acquisition.Science (New York, N.Y.) · 2016Article
- Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening.Microbial biotechnology · 2015Review
- Artificial cell-cell communication as an emerging tool in synthetic biology applications.Journal of biological engineering · 2015Article
- Permanent genetic memory with >1-byte capacity.Nature methods · 2014Article
- Phosphate sink containing two-component signaling systems as tunable threshold devices.PLoS computational biology · 2014Article
- Digital and analog gene circuits for biotechnology.Biotechnology journal · 2014Review
- Principles of genetic circuit design.Nature methods · 2014Review
- How to make a synthetic multicellular computer.PloS one · 2014Article
- Biological signal processing with a genetic toggle switch.PloS one · 2013Article
- Multicellular computing using conjugation for wiring.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Many applications require cells to switch between discrete phenotypic states. Here, we harness the FimBE inversion switch to flip a promoter, allowing expression to be toggled between two genes oriented in opposite directions. The response characteristics of the switch are characterized using two-color cytometry. This switch is used to toggle between orthogonal chemosensory pathways by controlling the expression of CheW and CheW*, which interact with the Tar (aspartate) and Tsr* (serine) chemoreceptors, respectively. CheW* and Tsr* each contain a mutation at their protein-protein interface such that they interact with each other. The complete genetic program containing an arabinose-inducible FimE controlling CheW/CheW* (and constitutively expressed tar/tsr*) is transformed into an Escherichia coli strain lacking all native chemoreceptors. This program enables bacteria to swim toward serine or aspartate in the absence or in the presence of arabinose, respectively. Thus, the program functions as a multiplexer with arabinose as the selector. This demonstrates the ability of synthetic genetic circuits to connect to a natural signaling network to switch between phenotypes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.