ArticleCurrent biology : CB2011
ATP hydrolysis is required for relocating cohesin from sites occupied by its Scc2/4 loading complex.
Article in Current biology : CB, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
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116 citing papers in PubMed, 195 citations in OpenAlex.
- Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia.Molecular cell · 2026Article
- Organization of replicated chromosomes by DNA loops and sister chromatid cohesion.Nature reviews. Molecular cell biology · 2026Review
- NIPBL and STAG1 enable loop extrusion by providing differential DNA-cohesin affinity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Sororin is an evolutionary conserved antagonist of WAPL.Nature communications · 2024Article
- Cohesin contributes to transcriptional repression of stage-specific genes in the human malaria parasite.EMBO reports · 2023Article
- ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.Nucleic acids research · 2023Article
- Loops and the activity of loop extrusion factors constrain chromatin dynamics.Molecular biology of the cell · 2023Article
- DecreasingScience advances · 2022Article
- Smc5/6 silences episomal transcription by a three-step function.Nature structural & molecular biology · 2022Article
- DNA sliding and loop formation byBiochemistry and biophysics reports · 2022Article
- Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection.Life science alliance · 2022Article
- Article
- Disruption of NIPBL/Scc2 in Cornelia de Lange Syndrome provokes cohesin genome-wide redistribution with an impact in the transcriptome.Nature communications · 2021Article
- Folding of cohesin's coiled coil is important for Scc2/4-induced association with chromosomes.eLife · 2021Article
- Genome folding through loop extrusion by SMC complexes.Nature reviews. Molecular cell biology · 2021Review
- Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.Nature immunology · 2021Article
- STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancers.Life science alliance · 2020Article
- Cohesin Causes Replicative DNA Damage by Trapping DNA Topological Stress.Molecular cell · 2020Article
- Evolutionary repair: Changes in multiple functional modules allow meiotic cohesin to support mitosis.PLoS biology · 2020Article
56 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe Cohesin complex that holds sister chromatins together until anaphase is comprised of three core subunits: Smc1 and Smc3, two long-rod-shaped proteins with an ABC-like ATPase head (nucleotide-binding domain [NBD]) and a dimerization domain linked by a 50 nm long intramolecular antiparallel coiled-coil, and Scc1, an α-kleisin subunit interconnecting the NBD domains of Smc1 and Smc3. Cohesin's stable association with chromosomes is thought to involve entrapment of chromatin fibers by its tripartite Smc1-Smc3-Scc1 ring via a poorly understood mechanism dependent on a separate Scc2/4 loading complex. A key issue concerns where entrapment initially takes place: at sites where cohesin is found stably associated or at distinct "loading" sites from which it translocates.
resultsIn this study, we find transition state mutant versions (Smc1E1158Q and SmcE1155Q) defective in disengagement of their nucleotide binding domains (NBDs), unlike functional cohesin, colocalize with Scc2/4 at core centromeres, sites that catalyze wild-type cohesin's recruitment to sequences 20 kb or more away. In addition to Scc2/4, the unstable association of transition state complexes with core centromeres requires Scc1's association with Smc1 and Smc3 NBDs, ATP-driven NBD engagement, cohesin's Scc3 subunit, and its hinge domain.
conclusionWe propose that cohesin's association with chromosomes is driven by two key events. NBD engagement driven by ATP binding produces an unstable association with specific loading sites like core centromeres, whereas subsequent ATP hydrolysis triggers DNA entrapment, which permits translocation along chromatin fibers.
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