ArticlePlant cell reports2011
Sonication-assisted Agrobacterium rhizogenes-mediated transformation of Verbascum xanthophoeniceum Griseb. for bioactive metabolite accumulation.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 60 citations in OpenAlex.
- Enhancement of Rosmarinic Acid Production in Hairy Root Cultures ofInternational journal of molecular sciences · 2025Article
- The Influence of Basal Medium on Polyphenol Accumulation in Shoot Cultures ofMolecules (Basel, Switzerland) · 2024Article
- A Plant Model ofApplied biosciences · 2024Article
- Optimization of induction and hairy root culture establishment in two mullein species, Verbascum erianthum and Verbascum stachydiforme.Scientific reports · 2024Article
- Ultrasound-Assisted Extraction of Verbascoside fromACS omega · 2023Article
- Hairy Root Cultures as a Source of Phenolic Antioxidants: Simple Phenolics, Phenolic Acids, Phenylethanoids, and Hydroxycinnamates.International journal of molecular sciences · 2023Review
- Hairy root culture technology: applications, constraints and prospect.Applied microbiology and biotechnology · 2021Review
- Production of Encecalin in Cell Cultures and Hairy Roots ofMolecules (Basel, Switzerland) · 2020Article
- Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment.3 Biotech · 2015Article
- Plant biotechnology in support of the Millennium Goals II.Plant cell reports · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient protocol for the establishment of transformed root culture of Verbascum xanthophoeniceum using sonication-assisted Agrobacterium rhizogenes-mediated transformation is reported. Only 10 days after the inoculation with A. rhizogenes ATCC 15834 and 45 s ultrasound exposure, hairy roots appeared on 75% of the Verbascum leaves. Ten hairy root lines were isolated, although only half of them were free of bacterial contamination and started growing when excised from mother explants. The transgenic nature of the most vigorously growing hairy root clones (VX1 and VX6) was confirmed by polymerase chain reaction. Under submerged cultivation both hairy root clones accumulated high biomass amounts (12.8 and 14.3 g L(-1), respectively) and significant amounts of bioactive phenylethanoid glycoside verbascoside (over 6-times more than in mother plant leaves). LC-APCI-MS analyses confirmed verbascoside accumulation in hairy root clones along with three other phenylethanoid glycosides (forsythoside B, leucosceptoside B and martynoside) and an iridoid glycoside aucubin. This is the first report on the induction of hairy roots of Verbascum plants.
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