ArticleJournal of bacteriology2011
Escherichia coli exports cyclic AMP via TolC.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Effects of TolC on the pathogenicity of porcine extraintestinal pathogenicFrontiers in immunology · 2022Article
- Secrete or perish: The role of secretion systems inComputational and structural biotechnology journal · 2021Review
- Type 1 Does the Two-Step: Type 1 Secretion Substrates with a Functional Periplasmic Intermediate.Journal of bacteriology · 2018Review
- Large-Scale Functional Analysis of CRP-Mediated Feed-Forward Loops.International journal of molecular sciences · 2018Article
- PredCRP: predicting and analysing the regulatory roles of CRP from its binding sites in Escherichia coli.Scientific reports · 2018Article
- Type I Protein Secretion-Deceptively Simple yet with a Wide Range of Mechanistic Variability across the Family.EcoSal Plus · 2016Review
- Rethinking the roles of CRP, cAMP, and sugar-mediated global regulation in the Vibrionaceae.Current genetics · 2016Review
- Genomic and experimental evidence for multiple metabolic functions in the RidA/YjgF/YER057c/UK114 (Rid) protein family.BMC genomics · 2015Article
- A new regulatory mechanism for bacterial lipoic acid synthesis.MicrobiologyOpen · 2015Article
- Hierarchy of non-glucose sugars in Escherichia coli.BMC systems biology · 2014Article
- Cyclic-AMP and bacterial cyclic-AMP receptor proteins revisited: adaptation for different ecological niches.Current opinion in microbiology · 2014Review
- TolC promotes ExPEC biofilm formation and curli production in response to medium osmolarity.BioMed research international · 2014Article
- Biochemical and functional characterization of SpdA, a 2', 3'cyclic nucleotide phosphodiesterase from Sinorhizobium meliloti.BMC microbiology · 2013Article
- Reduction of cellular stress by TolC-dependent efflux pumps in Escherichia coli indicated by BaeSR and CpxARP activation of spy in efflux mutants.Journal of bacteriology · 2013Article
- Identification of transport proteins involved in free fatty acid efflux in Escherichia coli.Journal of bacteriology · 2013Article
- Alkaline pH Is a signal for optimal production and secretion of the heat labile toxin, LT in enterotoxigenic Escherichia coli (ETEC).PloS one · 2013Article
- Article
- Novel ATP-driven pathway of glycolipid export involving TolC protein.The Journal of biological chemistry · 2011Article
- TolC affects virulence gene expression in Vibrio cholerae.Journal of bacteriology · 2011Article
- Mechanism and Function of the Outer Membrane Channel TolC in Multidrug Resistance and Physiology of Enterobacteria.Frontiers in microbiology · 2011Article
Corrections and comments
- Retraction · 2015-11-18Error in Analyses · Error in Data ·
- Retracted
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Escherichia coli more than 180 genes are regulated by the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. However, more than 90% of cAMP that is made by intracellular adenylyl cyclases is found in the culture medium. How is cAMP exported from E. coli? In a tolC mutant, 0.03 mM IPTG (isopropyl-β-d-thiogalactopyranoside) was sufficient to induce β-galactosidase compared to 0.1 mM IPTG in the parent strain. In a cya mutant unable to produce cAMP about 1 mM extracellular cAMP was required to induce β-galactosidase, whereas in a cya tolC mutant 0.1 mM cAMP was sufficient. When cAMP in E. coli cya was generated intracellularly by a recombinant, weakly active adenylyl cyclase from Corynebacterium glutamicum, the critical level of cAMP necessary for induction of maltose degradation was only achieved in a tolC mutant and not in the parent strain. Deletion of a putative cAMP phosphodiesterase of E. coli, CpdA, resulted in a slightly similar, yet more diffuse phenotype. The data demonstrate that export of cAMP via TolC is a most efficient way of E. coli to lower high concentrations of cAMP in the cell and maintain its sensitivity in changing metabolic environments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.