ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2011
Association of PDZ-containing protein PDZD11 with the human sodium-dependent multivitamin transporter.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Evaluation of PDZD11 in hepatocellular carcinoma: prognostic value and diagnostic potential in combination with AFP.Frontiers in oncology · 2025Article
- Review
- Identification ofInternational journal of general medicine · 2024Article
- Calsyntenin-3 interacts with the sodium-dependent vitamin C transporter-2 to regulate vitamin C uptake.International journal of biological macromolecules · 2021Article
- PLEKHA5, PLEKHA6, and PLEKHA7 bind to PDZD11 to target the Menkes ATPase ATP7A to the cell periphery and regulate copper homeostasis.Molecular biology of the cell · 2021Article
- Elevated Expression of PDZD11 Is Associated With Poor Prognosis and Immune Infiltrates in Hepatocellular Carcinoma.Frontiers in genetics · 2021Article
- Identification of transmembrane protein 237 as a novel interactor with the intestinal riboflavin transporter-3 (RFVT-3): role in functionality and cell biology.American journal of physiology. Cell physiology · 2019Article
- Identification and targeted management of a neurodegenerative disorder caused by biallelic mutations in SLC5A6.NPJ genomic medicine · 2019Article
- PLEKHA7 Recruits PDZD11 to Adherens Junctions to Stabilize Nectins.The Journal of biological chemistry · 2016Article
- The sodium/multivitamin transporter: a multipotent system with therapeutic implications.Vitamins and hormones · 2015Review
- Association of TM4SF4 with the human thiamine transporter-2 in intestinal epithelial cells.Digestive diseases and sciences · 2014Article
- Recent advances in transport of water-soluble vitamins in organs of the digestive system: a focus on the colon and the pancreas.American journal of physiology. Gastrointestinal and liver physiology · 2013Review
- Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- Effect of clinical mutations on functionality of the human riboflavin transporter-2 (hRFT-2).Molecular genetics and metabolism · 2012Article
- Tspan-1 interacts with the thiamine transporter-1 in human intestinal epithelial cells and modulates its stability.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Intestinal absorption of water-soluble vitamins in health and disease.The Biochemical journal · 2011Review
- Role of the putative N-glycosylation and PKC-phosphorylation sites of the human sodium-dependent multivitamin transporter (hSMVT) in function and regulation.Biochimica et biophysica acta · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Intestinal absorption of biotin is mediated via the sodium-dependent multivitamin transporter (SMVT). Studies from our laboratory and others have characterized different aspects of the human SMVT (hSMVT), but nothing is currently known about protein(s) that may interact with hSMVT and affect its physiology/biology. In this study, a PDZ-containing protein PDZD11 was identified as an interacting partner with hSMVT using yeast two-hybrid screen of a human intestinal cDNA library. The interaction between hSMVT and PDZD11 was confirmed by in vitro GST-pull-down assay and in vivo in a mammalian cell environment by a two-hybrid luciferase and coimmunoprecipitation assays. Furthermore, confocal imaging of live human intestinal epithelial HuTu-80 cells expressing hSMVT-GFP and DsRed-PDZD11 demonstrated colocalization of these two proteins. We also examined the functional consequence of the interaction between hSMVT and PDZD11 in HuTu-80 cells and observed significant induction in [(3)H]biotin uptake upon coexpression of hSMVT and PDZD11. In contrast, knocking down of PDZD11 with gene-specific small interfering RNA led to a significant decrease in biotin uptake; biotinylation assay showed this to be associated with a marked decrease in level of expression of hSMVT at the cell membrane. By truncation approach, we also demonstrated that the PDZ binding domain that is located in the COOH-terminal tail of hSMVT polypeptide is involved in the interaction with PDZD11. These results demonstrate for the first time that PDZD11 is an interacting partner with hSMVT in intestinal epithelial cells and that this interaction affects hSMVT function and cell biology.
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