ArticleApplied and environmental microbiology2011
Development and application of a method for counterselectable in-frame deletion in Clostridium perfringens.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
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- Development of a conditional plasmid for gene deletion in non-modelApplied and environmental microbiology · 2025Article
- Development of SacB-based counterselection for efficient allelic exchange inMicrobiology spectrum · 2025Article
- Purification of Type IV and Type VI Lipoteichoic Acids from Gram-Positive Mucosal Pathogens via a Two-Step Hot Water-Phenol/Triethylammonium Acetate Extraction Method.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Potent Bile Acid Microbial Metabolites ModulatePathogens (Basel, Switzerland) · 2023Article
- Genome-encoded ABCF factors implicated in intrinsic antibiotic resistance in Gram-positive bacteria: VmlR2, Ard1 and CplR.Nucleic acids research · 2023Article
- Article
- Holin-Dependent Secretion of the Large Clostridial Toxin TpeL by Clostridium perfringens.Journal of bacteriology · 2021Article
- A Putative Amidase Endolysin Encoded byAntibiotics (Basel, Switzerland) · 2021Article
- Plasmalogen Biosynthesis by Anaerobic Bacteria: Identification of a Two-Gene Operon Responsible for Plasmalogen Production inACS chemical biology · 2021Article
- Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens.NPJ biofilms and microbiomes · 2020Article
- Article
- An atypical lipoteichoic acid fromThe Journal of biological chemistry · 2020Article
- Changes in the expression of genes encoding type IV pili-associated proteins are seen when Clostridium perfringens is grown in liquid or on surfaces.BMC genomics · 2020Article
- CRISPR Genome Editing Systems in the GenusJournal of bacteriology · 2019Review
- Article
- Recent Developments of the Synthetic Biology Toolkit forFrontiers in microbiology · 2018Review
- Cyclic Di-GMP Binding by an Assembly ATPase (PilB2) and Control of Type IV Pilin Polymerization in the Gram-Positive Pathogen Clostridium perfringens.Journal of bacteriology · 2017Article
- Immunoactive Clostridial Membrane Vesicle Production Is Regulated by a Sporulation Factor.Infection and immunity · 2017Article
- Role of RNase Y in Clostridium perfringens mRNA Decay and Processing.Journal of bacteriology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many pathogenic clostridial species produce toxins and enzymes. To facilitate genome-wide identification of virulence factors and biotechnological application of their useful products, we have developed a markerless in-frame deletion method for Clostridium perfringens which allows efficient counterselection and multiple-gene disruption. The system comprises a galKT gene disruptant and a suicide galK plasmid into which two fragments of a target gene for in-frame deletion are cloned. The system was shown to be accurate and simple by using it to disrupt the alpha-toxin gene of the organism. It was also used to construct of two different virulence-attenuated strains, ΗΝ1303 and HN1314: the former is a disruptant of the virRS operon, which regulates the expression of virulence factors, and the latter is a disruptant of the six genes encoding the α, θ, and κ toxins; a clostripain-like protease; a 190-kDa secretory protein; and a putative cell wall lytic endopeptidase. Comparison of the two disruptants in terms of growth ability and the background levels of secreted proteins showed that HN1314 is more useful than ΗΝ1303 as a host for the large-scale production of recombinant proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.