ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2011
Fluid shear stress primes mouse embryonic stem cells for differentiation in a self-renewing environment via heparan sulfate proteoglycans transduction.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A quantitative meta-analysis comparing cell models in perfused organ on a chip with static cell cultures.Scientific reports · 2023Pooled it
- Simple 3D-printed stirred bioreactor enhances retinal organoid production via improved oxygenation.Cell reports methods · 2026Article
- Simple 3D-Printed Stirred Bioreactor Enhances Retinal Organoid Production Via Improved Oxygenation.bioRxiv : the preprint server for biology · 2025Article
- Article
- Dynamic in vitro culture of bovine and human ovarian tissue enhances follicle progression and health.Scientific reports · 2023Article
- Epigenetic Modification Factors and microRNAs Network Associated with Differentiation of Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Cardiomyocytes: A Review.Life (Basel, Switzerland) · 2023Review
- Using Live Imaging and Fluorescence Ubiquitinated Cell Cycle Indicator Embryonic Stem Cells to Distinguish G1 Cell Cycle Delays for General Stressors like Perfluoro-Octanoic Acid and Hyperosmotic Sorbitol or G2 Cell Cycle Delay for Mutagenic Stressors like Benzo(a)pyrene.Stem cells and development · 2022Article
- Mitochondria dysfunction in circulating tumor cells.Frontiers in oncology · 2022Review
- Extracellular Fluid Flow Induces Shallow Quiescence Through Physical and Biochemical Cues.Frontiers in cell and developmental biology · 2022Article
- Effects of shear stress on differentiation of stem cells into endothelial cells.World journal of stem cells · 2021Review
- Endothelial mechanotransduction in cardiovascular development and regeneration: emerging approaches and animal models.Current topics in membranes · 2021Review
- Microfluidic Systems with Embedded Cell Culture Chambers for High-Throughput Biological Assays.ACS applied bio materials · 2020Article
- Cell patterning by surface tension pinning in microfluidic channels.Biomicrofluidics · 2020Article
- Shear stress activates ATOH8 via autocrine VEGF promoting glycolysis dependent-survival of colorectal cancer cells in the circulation.Journal of experimental & clinical cancer research : CR · 2020Article
- Emerging Methods for Enhancing Pluripotent Stem Cell Expansion.Frontiers in cell and developmental biology · 2020Review
- Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis.World journal of stem cells · 2019Review
- User-defined morphogen patterning for directing human cell fate stratification.Scientific reports · 2019Article
- Environmental Specification of Pluripotent Stem Cell Derived Endothelial Cells Toward Arterial and Venous Subtypes.Frontiers in bioengineering and biotechnology · 2019Review
- Caring for cells in microsystems: principles and practices of cell-safe device design and operation.Lab on a chip · 2018Review
- A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation.Nature communications · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Shear stress is a ubiquitous environmental cue experienced by stem cells when they are being differentiated or expanded in perfusion cultures. However, its role in modulating self-renewing stem cell phenotypes is unclear, since shear is usually only studied in the context of cardiovascular differentiation. We used a multiplex microfluidic array, which overcomes the limitations of macroperfusion systems in shear application throughput and precision, to initiate a comprehensive, quantitative study of shear effects on self-renewing mouse embryonic stem cells (mESCs), where shear stresses varying by >1000 times (0.016-16 dyn/cm(2)) are applied simultaneously. When compared with static controls in the presence or absence of a saturated soluble environment (i.e., mESC-conditioned medium), we ascertained that flow-induced shear stress specifically up-regulates the epiblast marker Fgf5. Epiblast-state transition in mESCs involves heparan sulfate proteoglycans (HSPGs), which have also been shown to transduce shear stress in endothelial cells. By disrupting (with sulfation inhibitors and heparinase) and partially reconstituting (with heparin) HSPG function, we show that mESCs also mechanically sense shear stress via HSPGs to modulate Fgf5 expression. This study demonstrates that self-renewing mESCs possess the molecular machinery to sense shear stress and provides quantitative shear application benchmarks for future scalable stem cell culture systems.
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