Evidence map›Paper›PMID 21183295›Full record

ArticleVeterinary microbiology2011

First morphological characterization of 'Candidatus Mycoplasma turicensis' using electron microscopy.

Barbara Willi, Kristina Museux, Marilisa Novacco, Elisabeth M Schraner, Peter Wild, Katrin Groebel, Urs Ziegler, Godelind A Wolf-Jäckel, Yvonne Kessler, Catrina Geret and 3 more

Open access · hybridAbstract read
In one paragraph

Article in Veterinary microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Molecular characterization and phylogenetic analysis of feline hemoplasmas in domestic cats in Iran.Veterinary research forum : an international quarterly journal · 2017
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Barbara WilliClinical Laboratory, University of Zurich, Zurich, Switzerland. bwilli@vetclinics.uzh.ch
Kristina Museux
Marilisa Novacco
Elisabeth M Schraner
Peter Wild
Katrin Groebel
Urs Ziegler
Godelind A Wolf-Jäckel
Yvonne Kessler
Catrina Geret
Séverine Tasker
Hans Lutz
Regina Hofmann-Lehmann
University of Zurich · CHUniversity of Bristol · GB

Funding

Wellcome Trust WT077718
6 · The paper itself

Abstract

At least three haemotropic mycoplasmas have been recognized in cats: Mycoplasma haemofelis (Mhf), 'Candidatus Mycoplasma haemominutum' (CMhm) and 'Candidatus M. turicensis' (CMt). The latter was originally identified in a Swiss pet cat with haemolytic anaemia and shown to be prevalent in domestic cats and wild felids worldwide using molecular methods. So far, there has been no confirmatory morphological evidence of the existence of CMt presumably due to low blood loads during infection while CMhm has only been characterized by light microscopy with discrepant results. This study aimed to provide for the first time electron microscopic characteristics of CMt and CMhm and to compare them to Mhf. Blood samples from cats experimentally infected with CMt, CMhm and Mhf were used to determine copy numbers in blood by real-time PCR and for transmission and scanning electron microscopy. High resolution scanning electron microscopy revealed CMt and CMhm to be discoid-shaped organisms of 0.3 μm in diameter attached to red blood cells (RBCs). In transmission electron microscopy of CMt, an oval organism of about 0.25 μm with several intracellular electron dense structures was identified close to the surface of a RBC. CMhm and CMt exhibited similar morphology to Mhf but had a smaller diameter. This is the first study to provide morphological evidence of CMt thereby confirming its status as a distinct haemoplasma species, and to present electron microscopic features of CMhm.

Indexed as

AnimalsCat DiseasesCatsErythrocytesMicroscopy, Electron, ScanningMicroscopy, Electron, TransmissionMycoplasmaMycoplasma InfectionsReal-Time Polymerase Chain Reaction

Identifiers

PMID21183295
PMCPMC3127424
OpenAlexW2069915584

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.