ArticleMolecular and biochemical parasitology2011
Oral putrescine restores virulence of ornithine decarboxylase-deficient Leishmania donovani in mice.
Article in Molecular and biochemical parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Putrescine Depletion inPathogens (Basel, Switzerland) · 2025Article
- Polyamine Metabolism inMedical sciences (Basel, Switzerland) · 2022Review
- An overview of biochemically characterized drug targets in metabolic pathways of Leishmania parasite.Parasitology research · 2020Review
- Polyamines in protozoan pathogens.The Journal of biological chemistry · 2018Review
- Alpha-Difluoromethylornithine, an Irreversible Inhibitor of Polyamine Biosynthesis, as a Therapeutic Strategy against Hyperproliferative and Infectious Diseases.Medical sciences (Basel, Switzerland) · 2018Review
- Arginase Is Essential for Survival of Leishmania donovani Promastigotes but Not Intracellular Amastigotes.Infection and immunity · 2017Article
- Crystal structure of arginase from Leishmania mexicana and implications for the inhibition of polyamine biosynthesis in parasitic infections.Archives of biochemistry and biophysics · 2013Article
- Salmonella modulates metabolism during growth under conditions that induce expression of virulence genes.Molecular bioSystems · 2013Article
- The anti-trypanosome drug fexinidazole shows potential for treating visceral leishmaniasis.Science translational medicine · 2012Article
- Polyamine homoeostasis as a drug target in pathogenic protozoa: peculiarities and possibilities.The Biochemical journal · 2011Review
- Spermidine synthase is required for virulence of Leishmania donovani.Infection and immunity · 2011Article
- Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology.Enzyme research · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Administration of putrescine as a 1% solution in the drinking water ameliorated the profound loss of virulence exhibited by ornithine decarboxylase (ODC) deficient Leishmania donovani in mice. Furthermore, supplying α-difluoromethylornithine, an ODC inhibitor, at 2% in the drinking water reduced but did not eliminate infection with wild type L. donovani in the mouse model. Taken collectively, these findings: (1) demonstrate that oral putrescine can access the phagolysosome of macrophages in which the parasite resides in mice; (2) establish that the loss of virulence due to the Δodc lesion is a consequence of the inability of the mutant parasite to synthesize sufficient polyamines de novo; (3) imply that the L. donovani amastigote cannot access host polyamines in sufficient amounts for survival and growth; (4) and validate ODC as a drug target, although oral administration of DFMO is an unlikely therapeutic paradigm for visceral leishmaniasis.
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