ArticleBiochimica et biophysica acta2011
Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses.
Article in Biochimica et biophysica acta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 180 citations in OpenAlex.
- Evaluation of unipolar and bipolar nanosecond pulses for calcium electrochemotherapy and immune response.Frontiers in immunology · 2026Article
- Nanosecond pulsed electric fields induce cell-size-dependent selective permeabilization of urothelial cancer cells.Communications biology · 2025Article
- Live, spatially-resolved imaging of absolute intracellular water content using holotomography.Biomedical optics express · 2025Article
- The Influence of Moderate Electroporation onMicroorganisms · 2025Article
- Potentiation of Gelonin Cytotoxicity by Pulsed Electric Fields.International journal of molecular sciences · 2025Article
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- Uptake of substances into living mammalian cells by microwave induced perturbation of the plasma membrane.Scientific reports · 2024Article
- Nano-Pulse Stimulation Therapy Initiates Regulated Cell Death in Skin, Unlike Bovie Radiofrequency Ablation and Cryoablation.Bioelectricity · 2024Article
- Enhanced Cellular Doxorubicin Uptake via Delayed Exposure Following Nanosecond Pulsed Electric Field Treatment: An In Vitro Study.Pharmaceutics · 2024Article
- Nanosecond pulsed electric fields increase antibiotic susceptibility in methicillin-resistantMicrobiology spectrum · 2024Article
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- Dynamics of cell membrane lesions and adaptive conductance under the electrical stress.Cell stress · 2024Article
- Rapid and precise tracking of water influx and efflux across cell membranes induced by a pulsed electric field.Biomedical optics express · 2023Article
- Action spectra and mechanisms of (in) efficiency of bipolar electric pulses at electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
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- How to alleviate cardiac injury from electric shocks at the cellular level.Frontiers in cardiovascular medicine · 2022Article
- MLKL promotes cellular differentiation in myeloid leukemia by facilitating the release of G-CSF.Cell death and differentiation · 2021Article
- 10 ns PEFs induce a histological response linked to cell death and cytotoxic T-lymphocytes in an immunocompetent mouse model of peritoneal metastasis.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2021Article
- Single Cell Forces after Electroporation.ACS nano · 2021Article
- Incorporation ofFrontiers in microbiology · 2021Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Intense nanosecond-duration electric pulses (nsEP) open stable nanopores in the cell membrane, followed by cell volume changes due to water uptake or expulsion, as regulated by the osmolality balance of pore-impermeable solutes inside and outside the cell. The size of pores opened by either fifty 60-ns EP (~13 kV/cm) or five, 600-ns EP (~6 kV/cm) in GH3 cells was estimated by isoosmotic replacement of bath NaCl with polyethylene glycols and sugars. Such replacement reduced cell swelling or resulted in transient or sustained cell shrinking in response to EP. depending on the availability of pores permeable to the test solute. Unexpectedly, solute substitutions showed that for the same integral area of pores opened by 60- and 600-ns treatments (as estimated by cell volume changes), the pore sizes were similar. However, the 600-ns exposure triggered significantly higher cell uptake of propidium. We concluded that 600-ns EP opened a greater number of larger (propidium-permeable pores), but the fraction of the larger pores in the entire pore population was insufficient to contribute to cell volume changes. For both the 60- and 600-ns exposures, cell volume changes were determined by pores smaller than 0.9 nm in diameter; however, the diameter increased with increasing the nsEP intensity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.