ArticleWorld journal of gastroenterology2010
Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats.
Article in World journal of gastroenterology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Targeting the PI3K/AKT/mTOR pathway in gastrointestinal cancers: a comprehensive review of mechanisms, preclinical evidence, and clinical challenges.Medical oncology (Northwood, London, England) · 2026Review
- Young extracellular vesicles restore burn-induced adipose tissue immunometabolic and mitochondrial function in older mice.Scientific reports · 2025Article
- Mitochondrial Peroxiredoxins and Monoamine Oxidase-A: Dynamic Regulators of ROS Signaling in Cardioprotection.Physiological research · 2024Review
- Organismal metabolism regulates the expansion of oncogenic PIK3CA mutant clones in normal esophagus.Nature genetics · 2024Article
- Comparative mathematical modeling reveals the differential effects of high-fat diet and ketogenic diet on the PI3K-Akt signaling pathway in heart.Nutrition & metabolism · 2024Article
- The association of dietary insulinemic indices with PI3K, PTEN, and Akt gene expressions in visceral and subcutaneous adipose tissues among individuals undergoing abdominal surgery.Frontiers in nutrition · 2024Article
- Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Alpha-lipoic acid alleviates NAFLD and triglyceride accumulation in liver via modulating hepatic NLRP3 inflammasome activation pathway in type 2 diabetic rats.Food science & nutrition · 2021Article
- miR-122-5p/KIF5B/AMPK/AKT regulatory network regulates the progression of NAFLD.American journal of translational research · 2021Article
- Integrating Network Analysis and Metabolomics to Reveal Mechanism of Huaganjian Decoction in Treatment of Cholestatic Hepatic Injury.Frontiers in pharmacology · 2021Article
- Gut microbiota in atherosclerosis: focus on trimethylamine N-oxide.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2020Review
- Swimming Exercise Protects against Insulin Resistance via Regulating Oxidative Stress through Nox4 and AKT Signaling in High-Fat Diet-Fed Mice.Journal of diabetes research · 2020Article
- Gentiopicroside Ameliorates Oxidative Stress and Lipid Accumulation through Nuclear Factor Erythroid 2-Related Factor 2 Activation.Oxidative medicine and cellular longevity · 2020Article
- Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.Redox biology · 2018Article
- Differential effects of reticulophagy and mitophagy on nonalcoholic fatty liver disease.Cell death & disease · 2018Article
- HGF Secreted by Activated Kupffer Cells Induces Apoptosis ofFrontiers in immunology · 2017Article
- High-fat feeding does not induce an autophagic or apoptotic phenotype in female rat skeletal muscle.Experimental biology and medicine (Maywood, N.J.) · 2015Article
- Protective effects of phosphocreatine administered post-treatment combined with ischemic post-conditioning on rat hearts with myocardial ischemia/reperfusion injury.Journal of clinical medicine research · 2015Article
- Hind limb ischemia-reperfusion injury in diet-induced obese mice.The Journal of surgical research · 2014Article
- Adiponectin receptor 1 enhances fatty acid metabolism and cell survival in palmitate-treated HepG2 cells through the PI3 K/AKT pathway.European journal of nutrition · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimto determine whether mitochondrial dysfunction resulting from high-fat diet is related to impairment of the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt, also known as PKB) pathway.
methodsrat models of nonalcoholic fatty liver were established by high-fat diet feeding. The expression of total and phosphorylated P13K and Akt proteins in hepatocytes was determined by Western blotting. Degree of fat accumulation in liver was measured by hepatic triglyceride. Mitochondrial number and size were determined using quantitative morphometric analysis under transmission electron microscope. The permeability of the outer mitochondrial membrane was assessed by determining the potential gradient across this membrane.
resultsafter Wistar rats were fed with high-fat diet for 16 wk, their hepatocytes displayed an accumulation of fat (103.1 ± 12.6 vs 421.5 ± 19.7, P < 0.01), deformed mitochondria (9.0% ± 4.3% vs 83.0% ± 10.9%, P < 0.05), and a reduction in the mitochondrial membrane potential (389.385% ± 18.612% vs 249.121% ± 13.526%, P < 0.05). In addition, the expression of the phosphorylated P13K and Akt proteins in hepatocytes was reduced, as was the expression of the anti-apoptotic protein Bcl-2, while expression of the pro-apoptotic protein caspase-3 was increased. When animals were treated with pharmacological inhibitors of P13K or Akt, instead of high-fat diet, a similar pattern of hepatocellular fat accumulation, mitochondrial impairment, and change in the levels of PI3K, Akt, Bcl-2 was observed.
conclusionhigh-fat diet appears to inhibit the PI3K/Akt signaling pathway, which may lead to hepatocellular injury through activation of the mitochondrial membrane pathway of apoptosis.
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