ArticleProteomics2011
Identification of host factors involved in coronavirus replication by quantitative proteomics analysis.
Article in Proteomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Reconstructed signaling and regulatory networks identify potential drugs for SARS-CoV-2 infection.bioRxiv : the preprint server for biology · 2021Article
- Article
- Feline coronavirus replication is affected by both cyclophilin A and cyclophilin B.The Journal of general virology · 2017Article
- Article
- Profiling of Host Cell Response to Successive Canine Parvovirus Infection Based on Kinetic Proteomic Change Identification.Scientific reports · 2016Article
- Cellular peptidyl-prolyl cis/trans isomerase Pin1 facilitates replication of feline coronavirus.Antiviral research · 2016Article
- The proteome of the infectious bronchitis virus Beau-R virion.The Journal of general virology · 2015Article
- Article
- Looking for a needle in a haystack: Cellular proteins that may interact with the tyrosine-based sorting signal of the TGEV S protein.Virus research · 2015Review
- Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle.Methods in molecular biology (Clifton, N.J.) · 2015Article
- The Impact of Mass Spectrometry-Based Proteomics on Fundamental Discoveries in Virology.Annual review of virology · 2014Article
- Human coronavirus NL63 replication is cyclophilin A-dependent and inhibited by non-immunosuppressive cyclosporine A-derivatives including Alisporivir.Virus research · 2014Article
- Systems biology unravels interferon responses to respiratory virus infections.World journal of biological chemistry · 2014Review
- Magnetic fractionation and proteomic dissection of cellular organelles occupied by the late replication complexes of Semliki Forest virus.Journal of virology · 2013Article
- Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus.Proteome science · 2013Article
- Genome-wide gene-environment interaction analysis for asbestos exposure in lung cancer susceptibility.Carcinogenesis · 2012Article
- The interactome of the human respiratory syncytial virus NS1 protein highlights multiple effects on host cell biology.Journal of virology · 2012Article
- Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506.Virus research · 2012Article
- Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo.Proteome science · 2012Article
- Activity-based protein profiling of host-virus interactions.Trends in biotechnology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we applied a quantitative proteomic approach, based on SILAC, to investigate the interactions of coronaviruses with the secretory pathway of the host cell, with the aim to identify host factors involved in coronavirus replication. Comparison of the protein profiles of Golgi-enriched fractions of cells that were either mock infected or infected with mouse hepatitis virus revealed the significant depletion or enrichment of 116 proteins. Although ribosomal/nucleic acid binding proteins were enriched in the Golgi-fractions of mouse hepatitis virus-infected cells, proteins annotated to localize to several organelles of the secretory pathway were overrepresented among the proteins that were depleted from these fractions upon infection. We hypothesized that proteins, of which the abundance or distribution is affected by infection, are likely to be involved in the virus life cycle. Indeed, depletion of a small subset of the affected proteins by using small interfering RNAs identified several host factors involved in coronavirus infection. Transfection of small interfering RNAs targeting either C11orf59 or Golgi apparatus glycoprotein 1 resulted in increased virus replication, whereas depletion of vesicle-trafficking protein vesicle-trafficking protein sec22b enhanced the release of infectious progeny virus. Overexpression of these proteins, on the other hand, had a negative effect on virus replication. Overall, our study shows that the SILAC approach is a suitable tool to study host-pathogen interactions and to identify host proteins involved in virus replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.