ArticleMolecular biotechnology2011
Agrobacterium-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis L.
Article in Molecular biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Targeted genome modification in protoplasts of a tea cultivar Kolkhida using RNA-guided Cas9 endonuclease.AoB PLANTS · 2025Article
- The regulation of auxin receptor gene CsAFB2 by csn-miR393a confers resistance against Colletotrichum gloeosporioides in tea plants.Molecular plant pathology · 2025Article
- Article
- Understanding the Origin and Evolution of Tea (Camellia sinensis [L.]): Genomic Advances in Tea.Journal of molecular evolution · 2023Review
- Variation of Major Chemical Composition in Seed-Propagated Population of Wild Cocoa Tea PlantFoods (Basel, Switzerland) · 2022Article
- Comparative Transcriptome Analysis ofBiomolecules · 2022Article
- Review
- Tea plant genomics: achievements, challenges and perspectives.Horticulture research · 2020Review
- Biotechnological advances in tea (Camellia sinensis [L.] O. Kuntze): a review.Plant cell reports · 2016Review
- Article
- Enhanced resistance to blister blight in transgenic tea (Camellia sinensis [L.] O. Kuntze) by overexpression of class I chitinase gene from potato (Solanum tuberosum).Functional & integrative genomics · 2015Article
- Composite potato plants with transgenic roots on non-transgenic shoots: a model system for studying gene silencing in roots.Plant cell reports · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tea [Camellia sinensis (L.) O. Kuntze] is a perennial and most popular non-alcoholic caffeine-containing beverage crop. Tea has several constraints for its genetic improvement such as its high polyphenolic content and woody perennial nature. The development of transgenic tea is very difficult, laborious, and time taking process. In tea, regeneration requires minimum 8-12 months. In view of this, attempt has been made in this article to develop a rapid, efficient, and quite economical Agrobacterium-mediated root transformation system for tea. The feasibility of the developed protocol has been documented through silencing caffeine biosynthesis. For this, one-month-old tea seedlings were exposed to fresh wounding at the elongation zone of roots and were inoculated with Agrobacterium tumefaciens cultures carrying a RNAi construct (pFGC1008-CS). The pFGC1008-CS contained 376 bp of caffeine synthase (CS) cDNA fragment in sense and antisense direction with an intron in between. This has made the RNAi construct to produce a hairpin RNA (ihpRNA). The suppressed expression of CS gene and a marked reduction in caffeine and theobromine contents in young shoots of tea seedlings were obtained after root transformation through Agrobacterium infiltration. Such transformation system could be useful for functional analysis of genes in tea like woody and perennial plants.
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