ArticlePloS one2010
A transient transgenic RNAi strategy for rapid characterization of gene function during embryonic development.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Highly efficient and low-mosaicismiScience · 2026Article
- Development of a rapamycin-inducible protein-knockdown system in the unicellular red alga Cyanidioschyzon merolae.Plant physiology · 2024Article
- PRDM16 regulates a temporal transcriptional program to promote progression of cortical neural progenitors.Development (Cambridge, England) · 2021Article
- The Role of Reward System in Dishonest Behavior: A Functional Near-Infrared Spectroscopy Study.Brain topography · 2021Article
- Role of epigenetics and miRNAs in orofacial clefts.Birth defects research · 2020Review
- Multiple renal cyst development but not situs abnormalities in transgenic RNAi mice against Inv::GFP rescue gene.PloS one · 2014Article
- Redundant roles of PRDM family members in zebrafish craniofacial development.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
- Palatogenesis: morphogenetic and molecular mechanisms of secondary palate development.Development (Cambridge, England) · 2012Review
- Unique alterations of an ultraconserved non-coding element in the 3'UTR of ZIC2 in holoprosencephaly.PloS one · 2012Article
- Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
RNA interference (RNAi) is a powerful strategy for studying the phenotypic consequences of reduced gene expression levels in model systems. To develop a method for the rapid characterization of the developmental consequences of gene dysregulation, we tested the use of RNAi for "transient transgenic" knockdown of mRNA in mouse embryos. These methods included lentiviral infection as well as transposition using the Sleeping Beauty (SB) and PiggyBac (PB) transposable element systems. This approach can be useful for phenotypic validation of putative mutant loci, as we demonstrate by confirming that knockdown of Prdm16 phenocopies the ENU-induced cleft palate (CP) mutant, csp1. This strategy is attractive as an alternative to gene targeting in embryonic stem cells, as it is simple and yields phenotypic information in a matter of weeks. Of the three methodologies tested, the PB transposon system produced high numbers of transgenic embryos with the expected phenotype, demonstrating its utility as a screening method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.