ArticlePloS one2010
High diversity of the fungal community structure in naturally-occurring Ophiocordyceps sinensis.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 90 citations in OpenAlex.
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- Molecular phylogeny and morphology reveal two new species,MycoKeys · 2026Article
- Phytomycobiomes and Ecosystem Services: Mechanisms, Evidence and Routes to Application.Journal of fungi (Basel, Switzerland) · 2025Review
- Insights into the Mycosphere Fungal Community and Its Association with Nucleoside Accumulation inJournal of fungi (Basel, Switzerland) · 2025Article
- Compositional and functional diversities of core microbial communities in wild and artificial Ophiocordyceps sinensis.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Potential bioactivities via anticancer, antioxidant, and immunomodulatory properties of cultured mycelial enriched β-D-glucan polysaccharides from a novel fungusFrontiers in immunology · 2023Article
- Molecular-Based Diversity Studies and Field Surveys Are Not Mutually Exclusive: On the Importance of Integrated Methodologies in Mycological Research.Frontiers in fungal biology · 2022Article
- Endogenous bacteria inhabiting the Ophiocordyceps highlandensis during fruiting body development.BMC microbiology · 2021Article
- Complex microbial communities inhabiting natural Cordyceps militaris and the habitat soil and their predicted functions.Antonie van Leeuwenhoek · 2021Article
- Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the PredominantFrontiers in microbiology · 2021Article
- Different Cultivation Environments Affect the Yield, Bacterial Community and Metabolites ofFrontiers in microbiology · 2021Article
- Uncovering fungal community composition in natural habitat of Ophiocordyceps sinensis using high-throughput sequencing and culture-dependent approaches.BMC microbiology · 2020Article
- Article
- Infection ofFrontiers in microbiology · 2020Article
- Diversity and Co-Occurrence Patterns of Soil Bacterial and Fungal Communities of ChineseMicroorganisms · 2019Article
- Composition and predictive functional analysis of bacterial communities inhabiting Chinese Cordyceps insight into conserved core microbiome.BMC microbiology · 2019Article
- Current insights into fungal species diversity and perspective on naming the environmental DNA sequences of fungi.Mycology · 2019Review
- Fungus-larva relation in the formation of Cordyceps sinensis as revealed by stable carbon isotope analysis.Scientific reports · 2017Article
- Isolation, Culture and Characterization of Hirsutella sinensis Mycelium from Caterpillar Fungus Fruiting Body.PloS one · 2017Article
- Potential molecular mechanisms for fruiting body formation of Cordyceps illustrated in the case ofMycology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOphiocordyceps sinensis (syn. Cordyceps sinensis), which is a parasite of caterpillars and is endemic to alpine regions on the Tibetan Plateau, is one of the most valuable medicinal fungi in the world. "Natural O. sinensis specimens" harbor various other fungi. Several of these other fungi that have been isolated from natural O. sinensis specimens have similar chemical components and/or pharmaceutical effects as O. sinensis. Nevertheless, the mycobiota of natural O. sinensis specimens has not been investigated in detail. METHODOLOGY/PRINCIPAL
findingsBased on the technique of PCR-single-strand conformation polymorphism (PCR-SSCP), the mycobiota of three different sections (stromata, sclerotia, and mycelial cortices) from natural O. sinensis specimens were investigated using both culture-dependent and -independent methods. For the culture-dependent method, 572 fungal strains were isolated, and 92 putative operational taxonomic units (OTUs) were identified from 226 sequenced strains with the threshold of 97%. For the culture-independent method, 490 fungal clones were identified from about 3000 clones of ITS fragments from the whole-community DNA; based on PCR-SSCP analyses, 266 of these clones were selected to be sequenced, and 118 putative OTUs were detected. The overwhelming majority of isolates/clones and OTUs were detected from mycelial cortices; only a few were detected from stromata and sclerotia. The most common OTUs detected with both methods belonged to Ascomycota; however, only 13 OTUs were detected simultaneously by both methods. Potential novel lineages were detected by each of the two methods. CONCLUSIONS/SIGNIFICANCE: A great number of fungal species present in the mycobiota of naturally-occurring O. sinensis specimens were detected, and many of them may represent undescribed lineages. That only a few of the same OTUs were detected by both methods indicated that different methods should be used. This study increased our understanding about the fungal community structure of this valuable medicinal herb.
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