ArticlePloS one2010
Enumeration of functional T-cell subsets by fluorescence-immunospot defines signatures of pathogen burden in tuberculosis.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 87 citations in OpenAlex.
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- Diagnostic accuracy ofFrontiers in immunology · 2024Article
- Cytokine upsurge among drug-resistant tuberculosis endorse the signatures of hyper inflammation and disease severity.Scientific reports · 2023Article
- Maintenance and recall of memory T cell populations against tuberculosis: Implications for vaccine design.Frontiers in immunology · 2023Review
- Endometrial cytokine expression from clinically suspected genital tuberculosis patients at tertiary care hospitals in Dhaka.Journal of clinical tuberculosis and other mycobacterial diseases · 2022Article
- Comparison of Lethal and Nonlethal Mouse Models ofTropical medicine and infectious disease · 2021Article
- Characterizing Early T Cell Responses in Nonhuman Primate Model of Tuberculosis.Frontiers in immunology · 2021Article
- Immune Biomarkers for Diagnosis and Treatment Monitoring of Tuberculosis: Current Developments and Future Prospects.Frontiers in microbiology · 2019Review
- Where is tuberculosis transmission happening? Insights from the literature, new tools to study transmission and implications for the elimination of tuberculosis.Respirology (Carlton, Vic.) · 2018Review
- Altered expression of antigen-specific memory and regulatory T-cell subsets differentiate latent and active tuberculosis.Immunology · 2018Article
- Changes in cytokine responses to TB antigens ESAT-6, CFP-10 and TB 7.7 and inflammatory markers in peripheral blood during therapy.Scientific reports · 2018Article
- Stratification of Latent Mycobacterium tuberculosis Infection by Cellular Immune Profiling.The Journal of infectious diseases · 2017Article
- Utility of Th1-cell immune responses for distinguishing active tuberculosis from non-active tuberculosis: A case-control study.PloS one · 2017Article
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- Flow Cytometric Characterization of Antigen-Specific T Cells Based on RNA and Its Advantages in Detecting Infections and Immunological Disorders.Critical reviews in immunology · 2016Article
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- Differences in antigen-specific CD4+ responses to opportunistic infections in HIV infection.Immunity, inflammation and disease · 2015Article
- Kinetics of Mycobacterium tuberculosis-specific IFN-γ responses and sputum bacillary clearance in HIV-infected adults during treatment of pulmonary tuberculosis.Tuberculosis (Edinburgh, Scotland) · 2015Observational
- Article
- Specific in vitro interferon-gamma and IL-2 production as biomarkers during treatment of chronic Q fever.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundIFN-γ and IL-2 cytokine-profiles define three functional T-cell subsets which may correlate with pathogen load in chronic intracellular infections. We therefore investigated the feasibility of the immunospot platform to rapidly enumerate T-cell subsets by single-cell IFN-γ/IL-2 cytokine-profiling and establish whether immunospot-based T-cell signatures distinguish different clinical stages of human tuberculosis infection.
methodsWe used fluorophore-labelled anti-IFN-γ and anti-IL-2 antibodies with digital overlay of spatially-mapped colour-filtered images to enumerate dual and single cytokine-secreting M. tuberculosis antigen-specific T-cells in tuberculosis patients and in latent tuberculosis infection (LTBI). We validated results against established measures of cytokine-secreting T-cells.
resultsFluorescence-immunospot correlated closely with single-cytokine enzyme-linked-immunospot for IFN-γ-secreting T-cells and IL-2-secreting T-cells and flow-cytometry-based detection of dual IFN-γ/IL-2-secreting T-cells. The untreated tuberculosis signature was dominated by IFN-γ-only-secreting T-cells which shifted consistently in longitudinally-followed patients during treatment to a signature dominated by dual IFN-γ/IL-2-secreting T-cells in treated patients. The LTBI signature differed from active tuberculosis, with higher proportions of IL-2-only and IFN-γ/IL-2-secreting T-cells and lower proportions of IFN-γ-only-secreting T-cells.
conclusionsFluorescence-immunospot is a quantitative, accurate measure of functional T-cell subsets; identification of cytokine-signatures of pathogen burden, distinct clinical stages of M. tuberculosis infection and long-term immune containment suggests application for treatment monitoring and vaccine evaluation.
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